摘要
目的:研究雷公藤双向固体发酵过程中化学成分的变化规律。方法:采用紫外分光光度法测定雷公藤双向发酵过程中总二萜和总生物碱含量;采用高效液相色谱法测定雷公藤双向发酵过程中雷公藤甲素和两个单体生物碱含量。结果:在发酵90d时,雷公藤总二萜发酵下降了46%而总生物碱含量仅下降约13%,雷公藤甲素含量降低了89.4%而wilforgine和wilforine含量降级幅度较小。结论:双向发酵过程中真菌对雷公藤生物碱含量影响较小,而对二萜类化合物含量影响较大。
The content of total diterpenoids and total alkaloids were determined by UV spectrophotometry and triptolide and two main alkaloids contents were assayed by HPLC in order to study on the changes of chemical constituents in Tripterygium wilfordii during the process of fermenting. The results showed that after materials being fermented for 90 days,the content of total diterpene and total alkaloids decreased by 46% and 13% ,respectively,at the same time,triptolide content decreased by 89. 4% ,while the contents of wilforgine and wilforine decreased by little. It were concluded that fungi have large influence on the content of total diterpene in T. wilfordii F and less influence on total alkaloid during bi-directional fermentation in this paper.
出处
《中国实验方剂学杂志》
CAS
北大核心
2010年第10期59-62,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家科技部国际合作项目(2006DFA33060)
江西省卫生厅中医药科研基金项目(2007A023)
江西省教育厅项目(GJJ08334)
关键词
双向固体发酵
雷公藤
生物碱
二萜
雷公藤甲素
Bi-directional fermentation
Tripterygium wilfordii
alkaloid
diterpene
triptolide
