摘要
本文于2020年7—9月采集雾天和晴天生物气溶胶样品,利用高通量测序和BugBase基因功能预测,评估了雾天细菌气溶胶群落特征及潜在健康风险。结果表明,雾天可操作分类单元(OTUs)、物种多样性及丰富度均低于晴天,组内亲缘复杂度高于晴天。罗尔斯通氏菌、短杆菌、丙酸菌和短波单胞菌是雾天优势细菌属,雾天微生物几乎来源于本土,与晴天的细菌在属水平上差异性较小,只是相对丰度有所不同。革兰氏阴性菌(85%)与革兰氏阳性菌(13%)在两组样品中差别不大,但雾天潜在致病性细菌相对丰度(73.42%)高于晴天(59.95%)。两种天气均检测出棒状杆菌、沙雷氏菌、葡萄球菌、奈瑟菌、不动杆菌和摩根氏菌6种人间传染微生物,雾天总相对丰度为6.51%,晴天为4.42%,其中棒状杆菌、沙雷氏菌和葡萄球菌在两种天气下相对丰度相差不大。奈瑟菌在晴天相对丰度较高,不动杆菌以及摩根氏菌在雾天相对丰度较高。结合基因预测结果和潜在病原体分析,雾天致病性以及传染性菌种更为丰富,健康风险更高。
The health risk effects of bioaerosols under different weather conditions have received increasing attention.The characteristics of bioaerosols in haze have been extensively studied,but reports on foggy days are relatively rare.The foggy and sunny bioaerosol samples were collected from July to September 2020,and high-throughput sequencing and BugBase gene function prediction were used to evaluate the characteristics of the foggy bacterial community and potential health risks.The results showed that the OTUs,species diversity and richness in foggy days were lower than those in sunny days,and the complexity of kinship within the group was greater than in sunny days.Ralstonia,Brevibacterium,Propionibacterium and Brevundomonas are the dominant bacterias in foggy weather.The microbes in foggy days are almost native,and they are almost the same as the bacterial genera in sunny days,but there are some differences in relative abundance.There was little difference between Gram-negative bacteria(85%)and Gram-positive bacteria(13%)in the two samples,and the relative abundance of potentially pathogenic bacteria was higher in foggy days(73.42%)than that in sunny days(59.95%).Six human-infectious microorganisms were detected in both weathers Corynebacterium,Serratia,Staphylococcus,Neisseria,Acinetobacter and Morganella.The total relative abundance of foggy days is 6.51%,and that of sunny days is 4.42%.Among them,the relative abundance of Corynebacterium,Serratia,and Staphylococcus was not much different under the two weathers.Neisseria has a higher content on sunny days,Acinetobacter and Morganella have higher relative abundance on foggy days.Comprehensive gene prediction results and potential pathogen analysis,foggy weather pathogenic and infectious bacteria are more abundant,and health risks are higher.
作者
张婷
王太恒
祁建华
李先国
张大海
Zhang Ting;Wang Taiheng;Qi Jianhua;Li Xianguo;Zhang Dahai(College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao 266100, China;The Key Laboratory of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, China)
出处
《中国海洋大学学报（自然科学版）》
CAS
CSCD
北大核心
2022年第3期117-123,共7页
Periodical of Ocean University of China
基金
国家自然科学基金项目(41775148)
中央高校基本科研业务费专项(201762006)资助。
