摘要
目的比较分析应用段木和木屑栽培的桑黄中水分、总灰分、酸不溶性灰分、浸出物、粗蛋白质、粗脂肪、粗纤维、总多糖、总黄酮、总三萜含量,麦角甾醇和麦角甾酮的含量差异,并研究其乙醇提取物的体外抗氧化活性。方法分别选取段木和木屑栽培的桑黄子实体,依据国家标准对其水分、总灰分、酸不溶性灰分、浸出物、粗蛋白质、粗脂肪、粗纤维的含量进行测定,通过紫外分光光度法、高效液相色谱法(high performance liquid chromatography,HPLC)测定总多糖、总黄酮、总三萜、麦角甾醇、麦角甾酮含量,采用1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基清除能力、2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐[2’-azinobis-(3-ethylbenzthiazoline-6-sulphonate),ABTS]自由基清除能力评价其乙醇提取物的抗氧化活性。结果不同栽培基质对上述13个指标均具有影响。段木栽培的桑黄中水分、总灰分、酸不溶性灰分、浸出物、粗蛋白质、粗脂肪、粗纤维、总多糖、总三萜、麦角甾酮含量均高于木屑栽培桑黄;木屑栽培的桑黄中总黄酮、麦角甾醇含量高于段木栽培的桑黄;段木栽培桑黄的抗氧化活性高于木屑栽培桑黄。结论段木培养基更适宜桑黄子实体中各种化学成分的积累,从而提高其抗氧化活性,代料栽培产桑黄子实体的培养基需进一步优化,研究结果为桑黄栽培基质的优化和桑黄饮品质量评价提供了科学的参考。
Objective To compare and analyze the contents of water,total ash,acid insoluble ash,extract,crude protein,crude fat,crude fiber,total polysaccharides,total flavonoids,total triterpenes,ergosterol and ergosterone in Sanghuangporus vaninii cultivated with duanmu and sawdust,and study the antioxidant activities of its ethanol extract in vitro.Methods The fruit bodies of Sanghuangporus vaninii cultivated by segment wood and sawdust were selected.According to national standards,the content of moisture,total ash,acid-insoluble ash,extract,crude protein,crude fat,and crude fiber were determined.The content of total polysaccharides,total flavonoids,total triterpenes,ergosterol,and ergosterone was determined by ultraviolet spectrophotometry and high performance liquid chromatography(HPLC).1,1-diphenyl-2-picrylhydrazyl(DPPH)free radical scavenging ability and 2’-azinobis-(3-ethylbenzthiazoline-6-sulphonate)(ABTS)free radical scavenging ability were used to evaluate the antioxidant activity of the ethanol extract.Results Different culture media had effects on the above 13 indexes.The content of water,ash,acid insoluble ash,extract,crude protein,crude fat,crude fiber,total polysaccharide,total triterpene,ergosterone in Sanghuangporus vaninii cultivated with duanmu was higher than those cultivated with sawdust;the content of total flavonoids,ergosterol in Sanghuangporus vaninii cultivated with sawdust was higher than that cultivated with duanmu;the antioxidant activity of Sanghuangporus vaninii cultivated with duanmu was higher than that cultivated with sawdust.Conclusion Duanmu culture medium is more suitable for the accumulation of various chemical components in Sanghuangporus vaninii fruiting body,so as to improve its antioxidant activity.The culture medium for substitute cultivation of Sanghuangporus vaninii fruiting body needs to be further optimized.The research results provide a scientific reference for the optimization of Sanghuangporus vaninii cultivation matrix and the quality evaluation of Sanghuangporus vaninii
作者
李小欢
谢远娇
王欢
李敬超
刘俊泽
胡力铭
王淑敏
LI Xiao-Huan;XIE Yuan-Jiao;WANG Huan;LI Jing-Chao;LIU Jun-Ze;HU Li-Ming;WANG Shu-Min(College of Medicine,Changchun University of Chinese Medicine,Changchun 130117,China;Ginseng Science Research Institute,Changchun University of Chinese Medicine,Changchun 130117,China)
出处
《食品安全质量检测学报》
CAS
北大核心
2021年第23期9184-9189,共6页
Journal of Food Safety and Quality
基金
吉林省教育厅科研课题项目(JJKH20210993KJ)。
关键词
杨树桑黄
段木栽培
木屑栽培
抗氧化活性
麦角甾醇
麦角甾酮
Sanghuangporus vaninii
duanmu cultivation
sawdust cultivation
antioxidant activities
ergosterol
ergosterone
