摘要
【背景】暗蓝色链霉菌WMF106对核桃黑斑病病原菌野油菜黄单胞菌(Xanthomonas campestris pv.campestris)有较好的抑制作用,优化其发酵培养基与发酵条件将为核桃黑斑病生防菌剂的制备与应用提供参考。【目的】优化核桃黑斑病生防菌株WMF106的发酵条件,测定其抑菌物质稳定性,并通过田间防效测定验证防治效果,为后期规模化发酵以及菌剂的开发奠定基础。【方法】采用单因素和正交试验优化菌株WMF106的发酵培养基和发酵条件,观察pH、温度、光照、储藏温度对抑菌物质稳定性的影响,了解抑菌物质的极性,测定发酵液的田间防效。【结果】优化的最佳培养基为酵母浸粉2.0 g/L、燕麦20.0 g/L、NaCl 0.5 g/L、K_(2)HPO_(4)0.5 g/L、Mg SO_(4)·7H_(2)O 0.5 g/L。发酵条件为起始pH 6.0、接种量8%、装液量150 mL/500 mL、发酵时间9 d、发酵温度28℃、转速180 r/min时,抑菌物质活性最强。抑菌物质耐碱但对酸性环境敏感,具有较好的热、光及储藏温度稳定性,经乙酸乙酯萃取可获得最多的抑菌物质。施药后对叶片和果实的防效分别超过65.33%和61.21%。【结论】确定了菌株WMF106的最佳发酵培养基和发酵条件,抑菌物质有较好的稳定性及田间防治效果,应用开发前景广阔。
[Background]As an important biocontrol actinomyces,Streptomyces caeruleatus WMF106 exhibits good antagonistic effect on the pathogen of walnut blight,Xanthomonas campestris pv.campestris.To promote the preparation and application of antimicrobial agent in the control of the disease,it is necessary to improve the biomass,antimicrobial activity and stability of antimicrobial active substance for strain WMF106.[Objective]The present study aimed to explore an optimal fermentation condition and evaluate the stability and field control effect of antimicrobial active substance for Streptomyces strain WMF106.This study will be benefit for formulating large-scale fermentation procedure and development of bioagent.[Methods]Single factor experiment and orthogonal tests were used to optimize the fermentation medium and conditions.The effects of pH,temperature,light,ultraviolet,storage temperature on the stability of active substances and the polarity nature was determined by the single factor method respectively.To evaluate the results of optimization,the methods of field trials on leaves and fruits were used.[Results]The optimum composition of the fermentation medium was yeast extract 2.0 g/L,octmeal 20.0 g/L,NaCl 0.5 g/L,K_(2)HPO_(4)0.5 g/L MgSO_(4)·7H_(2)O 0.5 g/L.The optimized fermentation condition was initial pH 6.0,inoculation amount 8%,medium volume 150 mL/500 mL,fermentation time 9 d,fermentation temperature 28℃ and rotate speed 180 r/min.The results of the fermentation broth stability showed that the fermentation broth was alkali resistant but sensitive to acidic environments and had good heat,light and temperature tolerance.The active substances that obtained by using ethyl acetate has the highest antibacterial activity.The control effects of the fermentation broth against the disease that infected leaves and fruits were more than 65.33%and 61.21%,respectively.[Conclusion]The optimal fermentation medium and conditions were obtained.Moreover,it is also concluded that the fermentation broth of strain WMF106 not o
作者
瞿佳
孙晓宇
陈锐
门欣
赵玲侠
宁硕瀛
QU Jia;SUN Xiaoyu;CHEN Rui;MEN Xin;ZHAO Lingxia;NING Shuoying(Microbiology Institute of Shaanxi,Xi’an 710043,Shaanxi,China;Shaanxi Institute of Zoology,Xi’an 710032,Shaanxi,China)
出处
《微生物学通报》
CAS
CSCD
北大核心
2022年第1期88-100,共13页
Microbiology China
基金
陕西省科学院科技计划(2018nk-09,2018nk-01)
陕西省自然科学基础研究计划(2020JQ-973)
陕西省重点研发计划(2020NY-038)。
关键词
核桃黑斑病
拮抗放线菌
发酵优化
抑菌物质
walnut blight
antagonistic actinomycetes
fermentation optimization
antibacterial substances
