摘要
Chronic wounds are a serious worldwide problem,which are often accompanied by wound infections.In this study,bacterial cellulose(BC)-based composites introduced with tannic acid(TA)and magnesium chloride(BC-TA-Mg)were fabricated for anti-biofilm activities.The prepared composites’surface properties,mechanical capacity,thermal stability,water absorption and retention property,releasing behavior,anti-biofilm activities and potential cytotoxicity were tested.Results showed that TA and MgCl_(2) particles closely adhered to the nanofibers of BC membranes,thus increasing surface roughness and hydrophobicity of the membranes.While the introduction of TA and MgCl_(2) did not influence the transparency of the membranes,making it beneficial for wound inspection.BC-TA and BC-TA-Mg composites displayed increased tensile strength and elongation at break compared to pure BC.Moreover,BC-TA-Mg exhibited higher water absorption and retention capacity than BC and BC-TA,suitable for the absorption of wound exudates.BC-TA-Mg demonstrated controlled release of TA and good inhibitory effect on both singly cultured Staphylococcus aureus and Pseudomonas aeruginosa biofilm and co-cultured biofilm of S.aureus and P.aeruginosa.Furthermore,the cytotoxicity grade of BC-TA-6Mg membrane was eligible based on standard toxicity classifications.These indicated that BC-TA-Mg is potential to be used as wound dressings combating biofilms in chronic wounds.
基金
supported by the National Natural Science Foundation of China(grant numbers 51973018,51773018,31700829 and 52063030)
Fundamental Research Funds for the Central Universities and the Youth Teacher International Exchange&Growth Program(grant number QNXM20210019)
Key Research and Development Projects of People’s Liberation Army(grant number BWS17J036)
Natural Science Foundation of Jiangxi Province of China(grant number 20192ACB20033).
