摘要
目的评价疟原虫胶体金快速检测试剂盒法(RDTs法)在疟疾诊断中的应用价值。方法收集加蓬弗朗斯维尔中加友谊医院2020年9月至2021年6月105例疑似疟疾患者(中国人25例,加蓬人80例)全血标本,应用RDTs法进行检测,以显微镜镜检法作为金标准,分析RDTs法诊断恶性疟、非恶性疟的灵敏度、特异度,以及2种检测方法的综合推广率及检测时间。结果显微镜镜检法检出阳性患者92例,阳性率为87.6%,RDTs法检出阳性患者91例,阳性率为86.7%。RDTs法检测恶性疟的灵敏度为100.0%,特异度为94.7%,检测非恶性疟的灵敏度为66.7%,特异度100.0%。RDTs法、显微镜镜检法检测结果比较,差异无统计学意义(P>0.05)。根据10项单因素指标比对分析,RDTs法的综合推广率[86.7%(26/30)]高于显微镜镜检法[63.3%(19/30)],显微镜镜检法检出时间[(40.0±5.2)min]长于RDTs法[(12.6±1.5)min],差异均有统计学意义(P<0.05)。结论RDTs法可作为显微镜镜检法的辅助检测工具,在医疗条件落后的非洲地区推广使用。
Objective To evaluate the diagnostic value of plasmodium colloidal gold rapid detection kit(RDTs)in malaria.Methods From September 2020 to June 2021,105 suspected malaria blood samples(25 Chinese and 80 Gabonese)were collected from China-Gabon Friendship Hospital of Franceville in Gabon.By using microscopy as the gold standard,the sensitivity and specificity of RDTs were analyzed,and the comprehensive extension rate and detection time of the two methods were observed.Results The positive rate were 87.6%in 92 patients by microscopy and 86.7%in 91 patients by RDTs.The sensitivity and specificity of RDTs were 100.0%and 94.7%for falciparum malaria,and 66.7%and 100.0%for non-falciparum malaria.There was no significant difference on detection results between RDTs and microscopy(P>0.05).According to the comparative analysis of 10 single factor indexes,the comprehensive promotion rate of RDTs method[86.7%(26/30)]was higher than that of microscope method[63.3%(19/30)],and the detection time of microscope method[(40.0±5.2)min]was longer than that of RDTs method[(12.6±1.5)min],the differences were statistically significant(P<0.05).Conclusion RDTs could be used as an auxiliary detection tool for microscopic examination in Africa where medical conditions are backward.
作者
李世佳
NGANIANGA Chailina
LI Shijia;NGANIANGA Chailina(Department of Clinical Laboratory,Tianjin Forth Central Hospital,Tianjin 300143,China;China-Gabon Friendship Hospital of Franceville,Franceville 3725,Gabon)
出处
《检验医学与临床》
CAS
2022年第3期364-366,共3页
Laboratory Medicine and Clinic
关键词
疟疾
疟原虫
快速检测
镜检法
加蓬
malaria
plasmodium
rapid detection
microscopic examination
Gabon
