摘要
为检验市场上鸽新城疫抗体ELISA检测试剂盒的准确性及可靠性,从新疆喀什地区规模化鸽养殖场及鸽养殖合作社采集192份肉鸽血样,同时用血凝及血凝抑制试验以及某品牌鸽新城疫抗体ELISA检测试剂盒进行抗体检测,并以前者的检测结果作为"金标准",对ELISA检测试剂盒的性能进行评价。结果显示:ELISA试剂盒的敏感性为12.3%,特异性为97.4%,阳性似然比为4.73,阴性似然比为0.90,符合率为29.1%,约登指数为0.097,ROC曲线下面积为0.635。结果表明,该ELISA试剂盒检测鸽新城疫抗体的能力较差。这提示养殖企业及实验室选择ELISA方法检测鸽新城疫抗体前,应先对试剂有效性进行科学评估。
In order to test the accuracy and reliability of an ELISA kit for antibody against pigeon Newcastle disease virus(NDV)on the market,192 blood samples were collected from the pigeons for consumption in intensive farms and breeding cooperatives in Kashgar,Xinjiang to detect relevant antibody simultaneously by using hemagglutination and hemagglutination inhibition(HI)test as well as an ELISA kit,and to evaluate the performance of the kit by taking the results obtained by the former method as a“gold standard”. The results showed that,for the kit,the sensitivity,specificity,positive likelihood ratio,negative likelihood ratio,coincidence rate,yoden index and area under the ROC curve were 12.3%,97.4%,4.73,0.90,29.1%,0.097 and 0.635,respectively. It was thereby concluded that the kit was poor to detect antibody against pigeon NDV,against which,the effectiveness of reagents should be scientifically evaluated prior to application of an ELISA kit in detecting the antibody by breeding enterprises and laboratories.
作者
陈诒伟
付小龙
张毅
艾尼瓦尔·苏甫尔
丁洁
苏战强
Chen Yiwei;Fu Xiaolong;Zhang Yi;Ainiwaer Sufuer;Ding Jie;Su Zhanqiang(College of Veterinary Medicine,Xinjiang Agricultural University,Urumqi,Xinjiang 830000,China;Kashgar Vocational and Technical College,Kashgar,Xinjiang 844000,China)
出处
《中国动物检疫》
CAS
2022年第2期87-91,共5页
China Animal Health Inspection
基金
2020年喀什地区科技计划项目(KS2020028)
自治区家禽产业技术体系项目(2021xjjq-z-05)。
