摘要
植保素为重要的植物抗病防御代谢物,其生物合成需多种氧化酶参与。为研究短链脱氢酶(short-chain dehydrogenase,SDR)基因在水稻植保素生物合成和胁迫响应中的关键作用,本研究从水稻基因组数据中得到与已报道的水稻SDR基因同源性较高的4个基因:OsSDR1、OsSDR2、OsSDR3、OsSDR4,利用RT-PCR技术成功克隆到这4个基因,并进行序列比对及系统进化分析;通过蛋白原核表达,除OsSDR1未检测到蛋白表达,OsSDR2、OsSDR3和OsSDR4均成功表达出蛋白;采用RT-qPCR检测4个基因的诱导表达模式,结果显示4个基因对逆境有不同的响应模式,表明其可能参与到不同的代谢途径。本研究为研究水稻中SDRs蛋白的功能,解析水稻植保素生物合成途径,探讨其在水稻抗逆中的作用提供了研究依据。
Phytoalexins are the important defense metabolites against pathogen infection,and various oxidases are involved in phytoalexin biosynthesis.To study the key role of short-chain dehydrogenase(SDR)genes in phytoalexin biosynthesis and stress reponse in rice,four SDR genes including OsSDR1,OsSDR2,OsSDR3,OsSDR4 were identified from rice genomic data through homology searching using the characterized rice SDR genes.These four genes were successfully cloned by using RT-PCR technology,and sequence alignment and phylogenetic analysis were performed.Through the prokaryotic expression of the protein,OsSDR2,OsSDR3 and OsSDR4 successfully expressed the protein,except that there was no protein expression in OsSDR1.RT-qPCR was used to detect the induced expression patterns of the four genes,indicating that they may be involved in different metabolic pathways.This study provides a research basis for studying the function of SDRs protein in rice,analyzing the biosynthesis pathway of phytoalexin in rice,and exploring its role in stress resistance of rice.
作者
谢欣
谌琴琴
王丽平
王强
Xie Xin;Shen Qinqin;Wang Liping;Wang Qiang(Institute of Ecological Agriculture,Sichuan Agricultural University,Chengdu,611130)
出处
《分子植物育种》
CAS
北大核心
2022年第1期7-14,共8页
Molecular Plant Breeding
关键词
短链脱氢酶
植保素
逆境
Short-chain dehydrogenases
Phytoalexins
Stress
