摘要
目的回顾性分析筛查年龄及采样孕周对双胎妊娠孕妇外周血胎儿游离DNA浓度的影响。方法以同期进行无创产前检测的单胎妊娠孕妇为对照,对双胎妊娠孕妇无创产前检测结果及数据进行分析,评价双胎妊娠孕妇的筛查年龄和采样孕周对胎儿游离DNA浓度的影响。结果双胎妊娠孕妇NIPT平均筛查年龄及采样孕周均小于单胎,二者筛查年龄分别为31.72±3.45岁和33.14±3.73岁(P<0.01);采样孕周分别为15.30±1.94周和16.19±2.94周(P<0.01)。各年龄组双胎和单胎妊娠孕妇的胎儿游离DNA浓度差异无统计学意义(P>0.05)。双胎妊娠孕妇胎儿游离DNA浓度在孕16~17周及18~19周显著高于单胎(P<0.05)。双胎妊娠孕妇首次采血建库检测成功率低于单胎,二者成功率分别为97.13%和99.07%(P<0.05)。结论双胎妊娠孕妇cfDNA个体差异较大,可根据其临床指征,对双胎妊娠孕妇的NIPT筛查年龄及孕周做出适宜选择,适当提前NIPT筛查年龄、延后采样孕周,以减少因cfDNA浓度不足造成NIPT的检测失败。
Objective The effect of screening age and gestational weeks on plasma cell-free DNA concentration in pregnant women with twin pregnancy was retrospectively analyzed.Methods The results and data of noninvasive prenatal testing(NIPT)were analyzed with single pregnant women who underwent NIPT during the same period as the control group.The effects of age and sampling gestational weeks of the pregnant women on cell-free DNA concentration in the peripheral blood of pregnant women were evaluated.Results The mean detection age and sampling gestational weeks of NIPT in twins were lower than those in single pregnancies(31.72±3.45 years,33.14±3.73 years,respectively,P<0.01);Gestational weeks were 15.30±1.94 weeks and 16.19±2.94 weeks respectively(P<0.01).There was no significant difference in the concentration of cell-free DNA among the age groups.The cell-free DNA concentration of twin pregnant women was higher than that of single pregnant women at 16 to 17 gestational weeks and 18 to 19 gestational weeks(P<0.05).The success rate of the first database construction in the twins was lower than that in the single pregnancy(97.13%and 99.07%,respectively,P<0.05).Conclusion The NIPT screening age can be appropriately advanced and the gestational weeks of NIPT sampling of twin pregnant women can be appropriately postponed,in order to avoid the failure of detection due to the insufficient concentration of cell-free DNA.
作者
李珊珊
张萌
王威
王昕
陈玉娇
阴赪宏
王一鹏
LI Shanshan;ZHANG Meng;WANG Wei(Prenatal Diagnositic Center,Beijing Obstetrics and Gynecology Hospital,Capital Medical University,Beijing 100026,China)
出处
《医学研究杂志》
2022年第1期46-49,54,共5页
Journal of Medical Research
基金
国家重点研发计划项目(2016YFC1000101)。
关键词
双胎
无创产前检测
胎儿游离DNA浓度
Twin pregnancy
Noninvasive prenatal testing
Concentration of cell-free DNA
