摘要
目的探索具有癌痛特征的HCC患者血清硒含量特点,鉴定血清硒含量相关的lncRNA,并探讨血清硒含量与癌痛等级以及HCC预后的相关性。方法入组51例具有癌痛特征的HCC患者、51例良性肝病患者以及51例健康对照,采集其血样并进行血清硒含量检测。将HCC患者分为血清硒高含量组(Se_(high),27例)和血清硒低含量组(Se_(low),24例)。鉴定两组间的差异lncRNA,并统计患者血清硒含量与临床指征、预后指标以及癌痛等级的相关性。结果具有癌痛特征的HCC患者血清硒含量显著低于良性肝病组和健康对照组,且良性肝病组的血清硒含量显著低于健康对照组。Se_(high)组患者的癌痛等级显著低于Se_(low)组、而SAS评分显著高于Se_(low)组。血清硒含量与肿瘤患者的淋巴转移、肿瘤分期、生存时间等预后指标显著负相关。鉴定出一系列血清硒相关lncRNA,其中在Se_(high)组具有最高表达的是XLOC_019295,而表达最低的是XLOC_162298。结论具有癌痛特征的HCC患者血清硒含量与其癌痛等级和临床预后指标存在负相关性,这种功能可能由相关lncRNA介导,适当补硒对癌症发展和癌症相关疼痛具有抑制作用。
Objective To explore serum selenium content in hepatocellular carcinoma(HCC)patients,to identify selenium related lncRNA and to explore the correlation between serum selenium content and pain levels and prognosis.Methods Fifty-one HCC patients with cancer pain,together with 51 cases of benign liver disease patients and 51 cases of healthy controls were enrolled in this study.Serum selenium content was detected among these subjects.These patients were allocated into the Se_(high) group and Se_(low) group.The differentially expressed lncRNAs were identified between the two groups.The correlation between serum selenium content and clinical features,prognosis indicators and cancer pain levels were analyzed.Results Serum selenium content of HCC patients with cancer pain was significantly lower than that of benign liver disease patients and healthy controls,and serum selenium content of benign liver disease patients was significantly lower than that of healthy controls.Cancer pain levels of the serum selenium high group were significantly lower than those of the serum selenium low group,and serum selenium was negative related with the lymphatic metastasis,tumor stage,overall survival time and disease-free survival time.A series of serum selenium related lncRNAs were identified,among which XLOC_019295 was the highest expressed in the Se_(high) group,while XLOC_162298 was the lowest in the Se_(high) group.Conclusion There is a negative correlation between serum selenium content and pain levels and prognosis indicators in HCC patients with cancer pain,which might be mediated by certain lncRNAs.Our results suggest that appropriate selenium supplementation has a protective effect on postoperative pain.
作者
沙占明
丁修冬
SHA Zhanming;DING Xiudong(Department of Anesthesiology, The Third Hospital of Shandong Province, Jinan 250031, China;Department of Clinical Laboratory, Eighth Medical Center, PLA General Hospital, Beijing 100091, China)
出处
《标记免疫分析与临床》
CAS
2021年第11期1892-1897,1915,共7页
Labeled Immunoassays and Clinical Medicine
