摘要
建立了一种基于低共熔溶剂(DESs)的液液微萃取-高效液相色谱联用(LLME-HPLC)技术用于测定食用油中的双酚A(BPA)的方法,对比了3种DES和甲醇、乙二醇对食用油中BPA的萃取效率,研究了温度、萃取时间和萃取剂体积对萃取效率的影响。结果表明:氯化胆碱/乙二醇组成的DES萃取率最高;低共熔溶剂用量100μL,萃取温度25℃,萃取时间5 min时,萃取效率最高。BPA的检测限和定量限分别为0.10 mg/L和0.50 mg/L,加标回收率在86.3%~109.9%范围内,RSD小于6%。方法可用于食用油中BPA的检测。
A green solvent-deep eutectic solvents(DESs) and liquid-liquid microextraction(LLME) technology were employed in the extraction and determination of bisphenol A(BPA) from edible oils. The solvents including three DES, methanol and ethylene glycol on the extraction efficiency of BPA in edible oil were compared. The influence of temperature, extraction time and extraction volume were also investigated. The results showed that the DES composed of choline chloride and ethylene glycol(molar ratio 1:2) had the highest extraction efficiency than other two DESs of methanol and ethylene glycol, and the best extraction efficiency was obtained with DES volume of 100 μL, extraction temperature of 25℃ and extraction time of 5 min. After centrifugation, DES phase containing BPA was directly analyzed by high-performance liquid chromatograph-diode array detector(HPLC-DAD). The limits of detection and quantification(LOD and LOQ) were 0.10 mg/L and 0.50 mg/L, respectively. The average recoveries were 86.3%-109.9% and the relative standard deviations(RSDs) were below 6%. It was concluded that the method could be used for the detection of bisphenol A in edible oil.
作者
杨永超
杜宇
何成
于艳军
韩伟
李宁涛
熊中强
张颖
王利兵
YANG Yongchao;DU Yu;HE Cheng;YU Yanjun;HAN Wei;LI Ningtao;XIONG Zhongqiang;ZHANG Ying;WANG Libing(Technical Center for Safety of Industrial Products,Tianjin Customs,Tianjin 300308)
出处
《分析试验室》
CAS
CSCD
北大核心
2022年第1期33-37,共5页
Chinese Journal of Analysis Laboratory
基金
国家重点研发计划项目(2019YFC0810902
2020YFC0811100)和天津海关科研项目(2020THK011)资助。
关键词
绿色溶剂
低共熔溶剂
液液微萃取
食用油
双酚A
green solvent
deep eutectic solvents
liquid-liquid microextraction
edible oils
bisphenol A
