摘要
目的:建立高效液相色谱-紫外检测法测定零能量食用糖中三氯蔗糖含量的分析方法。方法:复配出的零能量食用糖经0.45μm的纤维素过滤膜净化,在流动相乙腈∶水体积比为12∶88,检测波长191 nm,柱温30℃的条件下经Agilent ZORBAX SB-C_(18)色谱柱(250 mm×4.6 mm,5μm)分离,紫外可见光检测器检测,外标法定量。结果:三氯蔗糖在100~2000μg/mL范围内呈良好的线性关系,相关系数r 2=0.9972。方法检出限为1.5μg/mL,定量限为5.0μg/mL。在浓度分别为10,20,50μg/mL加标水平下,三氯蔗糖的回收率为82.2%~107.6%,相对标准偏差为3.1%~5.9%(n=6)。结论:该方法简单快捷、便于操作、应用广泛、适用于普通实验室三氯蔗糖含量的测定。
Objective To establish an analytical method for the determination of sucralose in zero-energy edible sugar by high performance liquid chromatography-ultraviolet detection.The zero-energy edible sugar prepared by the method was purified by a 0.45μm cellulose filter membrane.The mobile phase was V(acetonitrile)∶V(water)=12∶88,the detection wavelength was 191 nm,and the column temperature was 30℃by Agilent ZORBAXSB-Separation on C_(18) chromatographic column(250 mm×4.6 mm,5μm),detection by UV-visible light detector,and quantification by internal standard method.Results Sucralose showed a good linear relationship in the range of 100~2000μg/L,and the correlation coefficient was r 2=0.9972.The detection limit of the method is 1.5μg/mL,and the limit of quantification is 5.0μg/mL.At the spiked levels of 10,20 and 50μg/L,the recovery rate of sucralose was 82.2%~107.6%,and the relative standard deviation was between 3.1%and 5.9%.Conclusion The method is simple,fast,easy to operate,widely used,and suitable for the determination of sucralose content in ordinary laboratories.
作者
梅梦迪
荣伟庆
付长亮
孟飞飞
鲁郑全
Mei Mengdi;Rong Weiqing;Fu Changliang;Meng Feifei;Lu Zhengquan(School of Chemical Engineering and Food Science,Zhengzhou University of Technology,Zhengzhou 450044,China)
出处
《山东化工》
CAS
2021年第24期128-130,共3页
Shandong Chemical Industry
基金
河南省高校重点科研项目(21B55007)资助。
关键词
三氯蔗糖
零能量食用糖
高效液相色谱
紫外可见光检测器
sucralose
zero-energy edible sugar
high performance liquid chromatography
ultraviolet detector
