摘要
目的观察雌激素受体拮抗剂对左归丸脑神经元保护作用的影响,初步探索中医学“肾脑相关”理论的物质基础。方法雌性SD大鼠108只,被随机分为6组,分别为假手术组(a组)、单纯脑缺血组(b组)、惊恐伤肾组(c组)、补肾中药组(d组)、雌激素受体拮抗剂组(e组)、脑复康组(f组),每组18只大鼠。除假手术组外,其余各组均用线栓法制备大鼠大脑中动脉阻塞(MCAO)模型。惊恐伤肾组、补肾中药组、雌激素受体拮抗剂组、脑复康组于MCAO手术前10 d进行高台恐吓,补肾中药组、雌激素受体拮抗剂组于MCAO前3 d开始分别给予左归丸汤剂1.62 g/kg灌胃,每日1次,连续给药10 d。脑复康组于MCAO前3 d开始分别给予脑复康悬液0.324 g/kg灌胃,每日1次,连续给药10 d。雌激素受体拮抗剂组于MCAO手术前3 d同时给予雌激素受体阻断剂ICI1827805 mg/kg腹腔注射,每日1次,连续给药10 d。采用平横木法检测各组大鼠的运动能力,新奇物认知法及跳台实验检测大鼠的记忆力,HE染色法观察大鼠脑组织形态学变化。结果与假手术组比较,单纯脑缺血组大鼠运动能力及记忆力均明显下降(P<0.01);与单纯脑缺血组比较,惊恐伤肾组大鼠运动能力及记忆力明显下降(P<0.05);与惊恐伤肾组比较,补肾中药组大鼠运动能力及记忆力明显提高(P<0.05);与补肾中药组比较,雌激素受体拮抗剂组大鼠运动能力及记忆力明显下降(P<0.05);与补肾中药组比较,脑复康组大鼠运动能力及记忆力无明显差异(P>0.05)。结论左归丸的脑神经元保护作用可以被雌激素受体拮抗剂阻断,提示左归丸通过雌激素及其受体对脑产生影响,“肾→雌激素及其受体→脑”可能是“肾通于脑”的关键通路之一。
Objective To observe the effect of estrogen receptor antagonist on the neuroprotective effect of Zuoguiwan Decoction,and explore the material basis of the theory of“kidney-brain connection”in traditional Chinese medicine.Methods A total of 108 female SD rats were randomly divided into 6 groups:sham operation group(group a),simple cerebral ischemia group(group b),panic injuring kidney group(group c),Chinese medicine kidney-tonifying group(group d),estrogen receptor antagonist group(group e),Naofukang group(group f),with 18 rats in each group.The middle cerebral artery occlusion(MCAO)model of rats was established by thread occlusion in all groups except sham operation group.Rats in group c,group d,group e and group f were threatened by high platform 10 days before MCAO.Rats in group d and group e were administered with Zuoguiwan Decoction 1.62 g/kg by gavage 3 days before MCAO,once a day for 10 consecutive days.Rats in group f were administered with Naofukang suspension 0.324 g/kg by gavage 3 days before MCAO,once a day for 10 consecutive days.Rats in group e were also administered with the estrogen receptor blocker ICI1827805 mg/kg intraperitoneally 3 days before MCAO,once a day for 10 consecutive days.The motor ability of rats in each group was measured by a horizontal beam method,the memory of rats was measured by a novel cognition method and platform jumping test,and the morphological changes of brain tissue were observed by HE staining method.Results Compared with group a,there was a significant decrease of the motor ability and memory of rats in group b(P<0.01);compared with group b,there was a significant decrease of the motor ability and memory of rats in group c(P<0.05);compared with group c,there was a significant improvement of the motor ability and memory of rats in group d(P<0.05);compared with group d,there was a significant decrease of the motor ability and memory of rats in group e(P<0.05);compared with group d,there was no significant difference of the motor ability and memory of rats in group
作者
刘宏
仲丽丽
张雨薇
杨婧
代巧妹
李冀
LIU Hong;ZHONG Lili;ZHANG Yuwei;YANG Jing;DAI Qiaomei;LI Ji(Department of Pathology,School of Basic Medical Sciences,Heilongjiang University of Chinese Medicine,Haerbin,Heilongjiang 150040,China;Postdoctoral Mobile Station,Heilongjiang University of Chinese Medicine,Haerbin,Heilongjiang 150040,China;Department of Pathology,The First Affiliated Hospital,Heilongjiang University of Chinese Medicine,Haerbin,Heilongjiang 150040,China;Department of Physiology,School of Basic Medical Sciences,Heilongjiang University of Chinese Medicine,Haerbin,Heilongjiang 150040,China;Department of Formulalogy,School of Basic Medical Sciences,Heilongjiang University of Chinese Medicine,Haerbin,Heilongjiang 150040,China)
出处
《上海中医药杂志》
2021年第11期66-73,共8页
Shanghai Journal of Traditional Chinese Medicine
基金
国家自然科学基金青年基金项目(81403288)
国家自然科学基金面上项目(82074288)
黑龙江省青年科学基金项目(QC2015102)
中国博士后科学基金面上资助项目(2016M591565)
哈尔滨市科技局青年后备人才计划项目(2016RAQXJ202)
黑龙江省博士后资助项目(LBH-Z15207)。
关键词
肾脑相关
雌激素
左归丸
大脑中动脉阻塞
雌激素受体拮抗剂
模型大鼠
中药研究
kidney-brain connection
estrogen
Zuoguiwan Decoction
MCAO
estrogen receptor antagonist
model rats
traditional Chinese herbal medicine research
