摘要
【目的】利用高效液相色谱法(HPLC)检测繁种用烟草绿原酸、芦丁、茄尼醇等重要活性成分的含量差异,为烟草种质资源的有效开发利用提供参考。【方法】采用HPLC法测定17份繁种用烟草种质资源成熟期叶片中绿原酸、芦丁、茄尼醇的含量,色谱条件为:Agilent 1260色谱仪,色谱柱为VP-ODS,流速1.0 mL/min,柱温35℃,检测器为1260 VWD。【结果】6种绿原酸异构体中,以绿原酸含量最高,在17份种质资源中均有检测到,且以S16打宾柳叶烟晒烟最高,其次是隐绿原酸和新绿原酸含量,而异绿原酸A、异绿原酸B和异绿原酸C的含量与前3种异构体相比,总体偏低;芦丁含量以NC347烤烟最高(46.456 g/kg),其次是S19大肩叶(7.782 g/kg)和S17大都青梗晒烟(6.250 g/kg),云烟87烤烟最低(1.206 g/kg);茄尼醇含量以NC347烤烟最高(44.913 g/kg),其次是K326烤烟(11.140 g/kg)、S17大都青梗晒烟(9.844 g/kg),黄坑7号-1烤烟最低(2.793 g/kg)。【结论】17份烟草种质资源中芦丁和茄尼醇的含量与绿原酸相比总体较高,且均以NC347烤烟最高。6种异构体中含量最高的是绿原酸,且以S16打宾柳叶烟晒烟最高。各繁种用烟草种质资源在现蕾后未打顶,这可能对绿原酸有较大影响。
【Objective】The contents of important active ingredients such as chlorogenic acid,rutin,and solanesol in different tobacco varieties for propagating were detected by HPLC method with a view to providing references for the effective development and utilization of tobacco germplasm resources.【Method】The contents of chlorogenic acid,rutin,and solanesol in leaves of 17 tobacco germplasm resources for propagating at mature stage were determined by HPLC.The chromatographic conditions were as follows:chromatograph was Agilent 1260,chromatographic column was VP-ODS,flow rate was 1.0 mL/min,the column temperature was 35℃and the detector was 1260 VWD.【Result】The content of chlorogenic acid was the highest among the six isomers,and was detected in all 17 germplasm resources,and that of S16 Dabinliuyeyan sun-cured tobacco was the highest,followed by cryptochlorogenic acid and neochlorogenic acid.Compared with the first three isomers,the content of isochlorogenic acid A,isochlorogenic acid B and isochlorogenic acid C was generally lower.The content of rutin in NC347 flue-cured tobac co was the highest(46.456 g/kg),followed by S19 Dajianye(7.782 g/kg)and S17 Daduqinggeng(6.250 g/kg)sun-cured tobaccos,and that of Yunyan 87 flue-cured tobacco was the lowest(1.206 g/kg).The content of solanesol in NC347 flue-cured tobacco was the highest(44.913 g/kg),followed by K326 flue-cured tobacco(11.140 g/kg)and S17 Daduqinggeng air-cured tobacco(9.844 g/kg),and that of Huangkeng No.7-1 flue-cured tobacco was the lowest(2.793 g/kg).【Conclusion】Among the 17 tobacco germplasm resources,the content of rutin and solanol was higher than that of chlorogenic acid,and the highest level was found in NC347 flue-cured tobacco.The content of chlorogenic acid was the highest among the six isomers,among which the S16 Dabinliuyeyan sun-cured tobacco had the highest content.The tobacco germplasm re sources for propagating did not topping after the appearance of flower-buds,which may have a greater effect on chlorogenic acid.
作者
邵秀红
马柱文
袁清华
李集勤
潘晓英
黄振瑞
SHAO Xiuhong;MA Zhuwen;YUAN Qinghua;LI Jiqin;PAN Xiaoying;HUANG Zhenrui(Crops Research Institute,Guangdong Academy of Agricultural Sciences/Guangdong Key Laboratory of Crops Genetics&Improvement/Guangdong Engineering&Technology Research Center for Tobacco Breeding and Comprehensive Utilization,Guangzhou 510640,China)
出处
《广东农业科学》
CAS
2021年第12期91-99,共9页
Guangdong Agricultural Sciences
基金
广东省农业科学院作物研究所所长基金(202002)
广东省烟草专卖局(公司)科技项目(粤烟科项201910)。
关键词
烟草
种质资源
活性成分
绿原酸
芦丁
茄尼醇
tobacco
germplasm resource
active ingredient
chlorogenic acid
rutin
solanesol
