摘要
城市河流承接不同类型的废水导致水质恶化,影响河流生态系统。通过分析河流细菌群落结构变化,不仅可以判断河流污染特征,还将有助于河流污染治理与生态修复。笔者研究了府河夏季上下游水体和沉积物理化性质,并采用高通量测序法分析了细菌群落结构特征。结果表明,府河上游水质化学需氧量(COD)、氨氮(NH_(3)-N)和总磷(TP)浓度显著高于下游(P<0.05),沉积物中重金属镉、锌和铅含量较高,均值分别为(0.44±0.03)、(182.17±0.34)和(35.76±0.20)mg·kg^(-1)。府河沉积物细菌多样性及丰富度明显高于相应上覆水体,变形菌门(Proteobacteria)是水体和沉积物中的第一优势菌,水体中参与氮循环的蓝藻菌门(Cyanobacteria)的高丰度说明府河存在一定的富营养化,此外,可以分解有机物的放线菌门(Actinobacteria)在下游丰度高于上游;沉积物中具有致病作用的拟杆菌门(Bacteroidetes)在上游城市河段丰度较高。细菌群落空间异质性结果表明下游硝化细菌(nitrifying bacteria)和反硝化细菌(denitrifying bacteria)的丰度(分别为62.25%和31.29%)明显比上游高。水体细菌多样性和温度、pH、总有机碳有显著相关性(P<0.05),冗余分析显示NH_(3)-N、总氮(TN)、TP和pH对细菌群落结构影响较大;而pH、TN和重金属镉是影响下游沉积物细菌群落结构的主要环境因子。
Different types of wastewater entering into urban rivers lead to the deterioration of river water quality and affect river ecosystem.Analyzing the changes in bacterial community structure of rivers can not only judge the characteristics of river pollution,but also help river pollution control and ecological restoration.In this paper,the physical and chemical properties of water and sediments of Fuhe River in summer were studied,and the characteristics of bacterial community structure were analyzed by high-throughput sequencing.The results showed that the concentrations of chemical oxygen demand(COD),ammonia nitrogen(NH_(3)-N),and total phosphorus(TP)in the upstream of Fuhe River were significantly higher than that in the downstream(P<0.05),and the contents of cadmium,zink and lead in the sediments were high with an average value of(0.44±0.03),(182.17±0.34)and(35.76±0.20)mg·kg^(-1),respectively.The bacterial diversity and richness in sediments of Fuhe River were significantly higher than those in the corresponding overlying water.Proteobacteria was the first dominant bacteria in the water and sediments.The high abundance of Cyanobacteria,which is involved in nitrogen cycle,indicated that there was certain eutrophication in Fuhe River.In addition,the abundance of Actinobacteria,which can decompose organic matters,was higher in the downstream than that in the upstream.The pathogenic Bacteroidetes was abundant in the upstream urban reach.The results of spatial heterogeneity of bacterial communities showed that nitrifying bacteria and denitrifying bacteria with a respective abundance of 62.25%and 31.29%were found to be higher in downstream than those in upstream.Correlation analysis showed that bacterial diversity was significantly correlated with temperature,pH,and total organic carbon(P<0.05).Redundancy analysis showed that NH_(3)-N,total nitrogen(TN),TP and pH had great impact on the bacterial community structure in water,while pH,TN and cadmium were the main environmental factors affecting the bacterial commun
作者
刘幸春
王洪杰
王亚利
王英俊
刘玲
Liu Xingchun;Wang Hongjie;Wang Yali;Wang Yingjun;Liu Ling(Xiong’an Institute of Eco-Environment,Hebei University,Baoding 071002,China;Institute of Life Science and Green Development,Hebei University,Baoding 071002,China;College of Life Sciences,Hebei University,Baoding 071002,China;Ecological Environment Bureau of Xiong’an New Area,Xiong’an New Area 071000,China)
出处
《生态毒理学报》
CAS
CSCD
北大核心
2021年第5期120-135,共16页
Asian Journal of Ecotoxicology
基金
国家水体污染控制与治理科技重大专项(2018ZX07110)
国家自然科学基金资助项目(51778054)。
关键词
府河
细菌
多样性
群落结构
环境因子
Fuhe River
bacteria
diversity
community structure
environmental factors
