摘要
以东亚飞蝗为材料筛选高效纤维素降解菌,富集培养后,以羧甲基纤维素钠为唯一碳源进行初筛,再通过刚果红鉴定和滤纸崩解实验复筛,并对小麦秸秆进行发酵实验,以验证其纤维素降解能力。获得了2株高效纤维素降解菌,分别为1号菌和4号菌,结合形态特征和分子生物学特征初步鉴定1号菌为灰色链霉菌,4号菌为短黄杆菌。结合细菌、真菌和放线菌在纤维素降解过程中扮演的不同角色,引入1株由真菌7号菌、1号菌和4号菌构建的高效纤维素降解复合菌系。红外测定和漆酶活性结果分析表明:复合菌系的综合降解效果最好,首先是纤维素被分解,此时纤维素酶活性最高;随后蜡质层表面逐渐脱落,木质素的苯环结构得以裸露,苯环的裂解使木质素进一步降解,此时漆酶活性较高。复合菌系小麦秸秆发酵培养7 d的降解率最高可达35%,有较高的研究价值。
In this paper,the Asiatic migratory locust was used as material to screen high-efficiency cellulose-degrading strains.After enrichment culture,sodium carboxy methylcellulose was used as the sole carbon source for preliminary screening.Then,Congo red identification and filter paper disintegration experiment were used to screen again.The wheat straw fermentation experiment was carried out to verify the cellulose degradation ability.Two high-efficiency cellulose-degrading bacteria were obtained,which were No.1 and No.4 bacteria.According to the characteristics of morphological and molecular biology,No.1 strain was identified as Streptomyces griseus and No.4 strain as Flavobacterium johnsoniae.Combined with the different roles of bacteria,fungi and actinomycetes in the process of cellulose-degradation,No.7 fungus,No.1 and No.4 bacteria were introduced to construct a high-efficiency cellulose-degradation composite microbial system.The results of FTIR and laccase activity analysis showed that the comprehensive degradation effect of the mixed strains was the best,the cellulose was decomposed firstly,and the cellulase activity was the highest at this time;then the surface of the wax layer gradually fell off,the benzene ring structure of lignin was exposed,and the decomposition of benzene ring made the lignin further degraded,and the laccase activity was higher at this time.Using composite strain,the degradation rate of wheat straw can reach up to 35%after fermentation culture for 7 days,which had great research value.
作者
金裕华
康薇
郑进
胡长洪
邹涛
JIN Yuhua;KANG Wei;ZHENG Jin;HU Changhong;ZOU Tao(School of Chemistry and Chemical Engineering,Hubei Polytechnic University,Huangshi Hubei 435003;.Hubei Key Laboratory of Mine Environmental Pollution Control and Remediation,Hubei Polytechnic University,Huangshi Hubei 435003)
出处
《湖北理工学院学报》
2022年第1期47-52,共6页
Journal of Hubei Polytechnic University
基金
湖北省中央引导地方科技发展专项(项目编号:2019ZYYD046)
湖北理工学院优秀青年科技创新团队资助计划项目(项目编号:13xtz08)
湖北理工学院引进人才项目(项目编号:13xjz04R)
矿区环境污染控制与修复湖北省重点实验室开放基金项目(项目编号:2014106)。
关键词
纤维素降解菌
筛选
降解效果
红外光谱
cellulose-degrading bacteria
screen
degradation effect
FTIR
