摘要
应用亲水性色谱法,建立了大黄鱼中ATP、ADP、AMP、IMP、HxR和Hx 6种关联物质的检测方法。以亲水性色谱柱BEH Amide为分析柱,2.0 mmol·L^(-1)磷酸二氢钾-乙腈为流动相,紫外检测进行定量分析。6种物质的方法检出限为0.1~0.4 mg·L^(-1),回收率为90.2%~106.3%。
A method for the determination of ATP, ADP, AMP, IMP, HxR and Hx in Pseudosciaena crocea was established by hydrophilic chromatography. The hydrophilic chromatographic column BEH-Amide was used as the analytical column and 2.0 mmol·L^(-1)potassium dihydrogen phosphate-acetonitrile was used as the mobile phase for quantitative analysis by UV detection.The detection limit of the six substances is 0.1~ 0.4 mg·L^(-1), and the recovery rate is 90.2%~ 106.3%.
作者
方周吉
张茹茹
余永康
蔡瑞鑫
张慧恩
FANG Zhouji;ZHANG Ruru;YU Yongkang;CAI Ruixin;ZHANG Hui’en(College of Biological and Environmental Sciences,Zhejiang Wanli University,Ningbo 315100,China)
出处
《现代食品》
2021年第20期184-187,共4页
Modern Food
基金
国家级大学生创新创业训练计划项目(202010876024)。
关键词
大黄鱼
亲水性色谱
ATP及关联产物
Pseudosciaena crocea
hydrophilic chromatography
ATP and related products
