摘要
建立了固相萃取结合光纤传感快速检测大鼠血浆中异丙酚的方法,并研究了异丙酚的药动学。采用正压固相萃取进行样品的快速前处理,构建光纤传感紫外检测系统,通过4-氨基安替比林衍生化法对样品进行衍生化后进行光纤传感紫外检测,并进行大鼠单次静脉注射异丙酚的药代动力学研究。该方法的线性范围为1.20~21.0μg/mL,定量限1.20μg/mL。异丙酚的回收率大于95%,RSD小于7%。大鼠单次静脉注射异丙酚后体内的代谢过程符合开放性二室模型,其分布半衰期(t_(1/2α))为1.335±0.052 min,消除半衰期(t_(1/2β))为50.09±0.016 min。血药浓度时间曲线下面积(AUC_((0-∞)))为212.65±39.4μg·min/mL。方法可用于异丙酚药代动力学研究和血药浓度检测。
A solid phase extraction method combined with fiber optic sensing for rapid detection of propofol in rat plasma was established,and its pharmacokinetics was studied.Firstly,the samples were pretreated with positive pressure solid-phase extraction.Secondly,a fiber optic sensing ultraviolet detection system was constructed,the samples were derivatized by 4-aminoantipyrine derivatization method and then detected by fiber optic sensing ultraviolet detection system.Finally,the pharmacokinetics of a single intravenous injection of propofol in rats was studied.The linear range of the this method was 1.20-21.0μg/mL,and the limit of quantification(LOQ)was 1.20μg/mL.The recovery of propofol was more than 95%and the standard deviation was less than 7%.The metabolic process in rats after a single intravenous injection of propofol conformed with the open two-compartment model,the distribution half-life(t_(1/2α))was 1.335±0.052 min,and the elimination half-life(t_(1/2β))was 50.09±0.016 min.The area under curve(AUC_((0-∞)))was 212.65±39.4μg·min/mL.The method of rapid detection of propofol by solid phase extraction combined with fiber optic sensing was suitable for pharmacokinetic study and determination of propofol in plasma.
作者
刁娟娟
李莉
安琪
王婷婷
DIAO Juanjuan;LI Li;AN Qi;WANG Tingting(Central Laboratory,Xinjiang Medical University,Urumqi 830011;College of Pharmacy,Xinjiang Medical University,Urumqi 830011)
出处
《分析试验室》
CAS
CSCD
北大核心
2021年第12期1376-1380,共5页
Chinese Journal of Analysis Laboratory
基金
国家自然科学基金项目(81760645)资助。
关键词
固相萃取
光纤传感
异丙酚
药动学
solid phase extraction
fiber optic sensing
propofol
pharmacokinetics
