摘要
为了降低纤维乙醇生产过程中纤维素酶成本,以高产纤维素酶里氏木霉TGC521为生产菌株,玉米秸秆汽爆料为碳源,对纤维素酶的发酵工艺进行优化研究。在单因素试验基础上,利用Box-Behnken设计和响应面方法对培养基配方进行优化,并研究了溶氧、还原糖等工艺参数对发酵产酶的影响以及所产纤维素酶的应用。结果表明,玉米秸秆汽爆料对产酶影响最大,其次为玉米浆。优化培养基配方为:玉米秸秆汽爆料38.6 g/L、(NH_(4))_(2)SO_(4)4.8 g/L、玉米浆29.7 g/L、麸皮5.3 g/L、KH_(2)PO_(4)1.0 g/L、MgSO_(4)0.5 g/L。在溶氧浓度20%~30%、还原糖含量2.0~3.0 g/L、发酵时间144 h条件下,纤维素酶活力达到39.0 U/mL。进一步利用生产的纤维素酶进行原位糖化,低酶载量条件下纤维素转化率达85%以上。
To reduce the cellulase cost in the production of cellulosic ethanol,Trichoderma reesei TG-C521 with high-yield cellulase was used with steam-exploded corn stover as the carbon source.The influence of dissolved oxygen and reducing sugar on the production and application of cellulase were studied.The results showed that the steam-exploded corn stover had the most significant effect on the cellulase production,followed by corn steep liquor.The optimal medium was as follows:38.6 g/L of steam-exploded corn stover,4.8 g/L of(NH_(4))_(2)SO_(4),29.7 g/L of corn steep liquor,5.3 g/L of bran,1.0 g/L of KH_(2)PO_(4),0.5 g/L of MgSO_(4).Under the conditions of the dissolved oxygen 20%-30%,reducing sugar 2.0-3.0 g/L and fermentation time 144 h,the cellulase activity reached 39.0 U/mL.The cellulase was further used for in situ saccharification,the cellulose conversion can reach more than 85%with low cellulase loading.
作者
王蓓
陈吉宝
阎振丽
赵子高
李超
WANG Bei;CHEN Jibao;YAN Zhenli;ZHAO Zigao;LI Chao(Agricultural Engineering,Nanyang Normal University,Nanyang 473000,China;Tianguan Nanyang Fiber Ethanol Co.Ltd.,Nanyang 473000,China;State Key Laboratory of Motor Vehicle Biofuel Technology,Henan Tianguan Group Co.Ltd.,Nanyang 473000,China)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2022年第1期182-187,共6页
Food and Fermentation Industries
基金
国家重点研发计划项目(2018YFB1501705-2)
南阳市协同创新重大专项(NRIZU2020CIP0006)。
关键词
纤维乙醇
玉米秸秆汽爆料
纤维素酶
补料发酵
原位水解糖化
cellulose ethanol
corn stalk steam explosion
cellulase
fed-batch fermentation
in situ saccharification
