摘要
目的制作一种用于小鼠后肢针灸的简易固定装置,为针灸实验操作提供经济实用、便利高效的工具。方法按照设计图制作小鼠后肢固定器,由4位实验员A、B、C、D分别使用布制鼠衣和小鼠后肢固定器固定小鼠,针刺后肢足三里穴,观察固定所需时间、小鼠后肢回缩次数、针具脱落次数、小鼠体重以比较二者在针刺操作中的固定效果及对小鼠应激反应的影响;此外通过热辐射痛阈检测、旷场实验、高架十字迷宫实验观察不同固定方法对小鼠痛阈、认知、情绪等的影响。结果小鼠后肢固定器制作简单,与布制鼠衣相比,对小鼠体重的影响无明显差别,但使用小鼠后肢固定器固定小鼠进行针刺效率更高,固定后小鼠后肢回缩次数及针具脱落次数较少;热辐射痛阈检测、旷场实验、高架十字迷宫实验各项指标组间无明显差异。结论小鼠后肢固定器使用方便,有助于提高实验效率,对小鼠痛阈、认知、情绪等无明显影响,可根据具体的实验需求使用。
Objective To manufacture a simple device for securing the hind limbs of mice for acupuncture and moxibustion treatment,and provide a convenient economical,efficient,and practical tool for acupuncture and moxibustion experiments.Methods A holding device for mouse hind limbs was made according to design drawings.Four investigators secured mice with specialized cloth(clothing method)or the mouse hind limb device,and then needled mice at the Zusanli point.To compare the effects of these two holding method in acupuncture,the holding time,number of hind limbs retracted,number of needles shed and the body weight of mice were recorded,and the influence on the stress response of mice observed.In addition,the effects of different holding method on pain threshold,cognition and emotion were observed by the thermal radiation pain threshold,open field and elevated cross maze tests.Results Compared with the clothing method,there was no significant difference in the body weight of mice with the holding device during acupuncture.The mouse hind limb holding device was easy to make,more efficient,and had less frequent hind limb retraction and needle shedding compared with the clothing method.There were no significant differences in the thermal radiation pain threshold,open field and elevated cross maze tests between the two holding method.Conclusions The hind limb holding device was convenient,which helped to improve the efficiency of the experiment,and had no significant impact on pain threshold,cognition and emotion compared with the clothing method.The holding device can be used for specific experimental needs.
作者
苑功名
刘兴星
公一囡
王洋洋
杨静雯
徐媛
郭永明
YUAN Gongming;LIU Xingxing;GONG Yinan;WANG Yangyang;YANG Jingwen;XU Yuan;GUO Yongming(Research Center of Experimental Acupuncture Science,Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China)
出处
《中国比较医学杂志》
CAS
北大核心
2021年第12期14-19,共6页
Chinese Journal of Comparative Medicine
基金
国家自然科学基金面上项目(81873368)
天津市高等学校科技发展基金项目(020327)。
关键词
针灸
小鼠
后肢
固定器
acupuncture and moxibustion
mice
hind limb
fixator
