摘要
通过超氧阴离子清除实验评估了炙甘草和当归中黄酮类成分配伍的抗炎活性及复配比例,利用正交试验优选凝胶的基质配方,建立小鼠深Ⅱ度烫伤模型,创面外用凝胶进行治疗,在烫伤不同时段测定小鼠组织含水量及创面愈合率,血清中TNF-α、IL-1以及组织中EGF、VEGF的含量;实验表明炙甘草和当归配比在1∶2时,对超氧阴离子的清除作用最强;凝胶基质中添加卡波姆1%、甘油5%、丙二醇10%时,凝胶的性状最佳,凝胶外用能显著提高小鼠创面愈合率,减轻组织水肿,降低组织含水量,减轻炎症反应,提高组织中EGF、VEGF含量,差异有统计学意义(P<0.05)。实验所制备的凝胶具有生肌止痛,抗炎消肿,对烫伤的治疗有良好作用。
The anti-inflammatory activity and compatibility ratio of flavonoids in Glycyrrhizae Radix et Rhizoma Praeparata Cum Melle(GR)and Angelicae Sinensis Radix(AS)were evaluated by the superoxide anion scavenging test.The matrix formula of gel was optimized by orthogonal test design and the model of deep partial-thickness scald in mice was induced.The gel was applied to the wound.The tissue water content,wound healing rate,serum TNF-αand IL-1,and EGF and VEGF in tissues were measured at different periods.The results revealed that when the compatibility ratio of GR and AS was 1∶2,the maximal scavenging efficacy on superoxide anion was observed.The gel displayed the optimal properties when carbomer(1%),glycerol(5%),propylene glycol(10%)were added into the matrix.Gel external application can significantly improve the wound healing rate,relieve tissue edema,diminish tissue water content,alleviate inflammatory reaction,and increase the content of EGF and VEGF in tissues(P<0.05).The gel prepared in the present study is effective in promoting granulation,relieving pain,resisting inflammation,and alleviating edema,and is potent in healing scalds.
作者
董蕊
王盼
逯影
DONG Rui;WANG Pan;LU Ying(College of Chinese Medicine Materials,Jilin Agricultural University,Changchun 130118,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2021年第22期5810-5818,共9页
China Journal of Chinese Materia Medica
基金
国家中药标准化项目(ZYBZH-Y-JL-25)。
关键词
炙甘草
当归
黄酮
凝胶
外用
烫伤
Glycyrrhizae Radix et Rhizoma Praeparata Cum Melle(GR)
Angelicae Sinensis Radix(AS)
flavonoids
gel
external application
scalds
