摘要
目的探讨金雀异黄酮(GEN)对大鼠视网膜缺血-再灌注(I/R)损伤的保护作用。方法选取30只健康SPF级大鼠,按照随机数字表法将其分为假手术组、I/R组和I/R+GEN组,每组各10只。I/R组和I/R+GEN组大鼠采用前房灌注生理盐水升高眼压法制备视网膜I/R损伤大鼠模型,I/R组大鼠术后每天给予注射生理盐水(1 mL·kg^(-1)·d^(-1));I/R+GEN组大鼠术后每天给予注射GEN(40 mL·kg^(-1)·d^(-1));假手术组大鼠行相应假手术后每天予以注射生理盐水(1 mL·kg^(-1)·d^(-1)),连续7 d后,颈椎脱臼法处死各组大鼠,取眼部组织。HE染色观察各组大鼠视网膜的形态以及内丛状层(IPL)、内核层(INL)和神经节细胞层(GCL)厚度;免疫荧光分析观察各组大鼠视网膜神经节细胞(RGC)的存活率;TUNEL染色检测各组大鼠视网膜细胞凋亡水平;检测各组大鼠视网膜组织中过氧化氢酶(CAT)、丙二醛(MDA)及超氧化物歧化酶(SOD)水平以反映视网膜氧化应激状态;Western blot分析三组大鼠视网膜中NLRP3、ASC、Caspase-1的蛋白表达。结果I/R组、I/R+GEN组和假手术组大鼠视网膜总厚度分别为(114.37±7.32)μm、(155.31±6.83)μm和(178.98±13.65)μm(t=14.284,P<0.01),I/R组低于假手术组和I/R+GEN组,差异均有统计学意义(均为P<0.01)。I/R组大鼠视网膜IPL、INL和GCL厚度分别为(18.95±5.06)μm、(17.62±4.69)μm和(19.03±4.74)μm,I/R+GEN组大鼠视网膜IPL、INL和GCL厚度分别为(20.69±8.13)μm、(25.74±6.78)μm和(26.71±7.85)μm,假手术组大鼠视网膜IPL、INL和GCL厚度分别为(11.73±3.15)μm、(11.97±3.56)μm和(12.59±3.24)μm(均为P<0.05),I/R组与假手术组和I/R+GEN组三个指标比较差异均有统计学意义(均为P<0.01)。I/R组、I/R+GEN组和假手术组大鼠视网膜中RGC相对存活率分别为(26.87±3.12)%、(73.46±7.80)%和(100.00±5.64)%(t=16.825,P<0.01),I/R组RGC相对存活率低于假手术组和I/R+GEN组,差异均有统计学意义(均为P<0.01)。I/R组、I/R+GEN组和假手�
Objective To explore the protective effect of genistein(GEN)on retinal ischemia-reperfusion(I/R)injury in rats.Methods Thirty healthy SPF rats were selected and randomly divided into the sham operation group,I/R group,and I/R+GEN group,with 10 rats in each group.In the I/R group and I/R+GEN group,I/R rat models were prepared by anterior chamber infusion of normal saline to increase intraocular pressure.In the I/R group,rats were injected with 1 mL·kg^(-1)·d^(-1)normal saline every day after operation.In the I/R+GEN group,rats were injected with 40 mL·kg^(-1)·d^(-1)GEN every day after operation.In the sham operation group,rats were injected with 1 mL·kg^(-1)·d^(-1)normal saline every day after the corresponding sham operation.Seven consecutive days later,all rats were dislocated and sacrificed,and their eye tissues were taken.The hematoxylin and eosin(HE)staining was used to observe the retinal morphology and thickness of inner plexiform layer(IPL),inner nuclear layer(INL)and ganglion cell layer(GCL).The immunofluorescence assay was used to determine the survival rate of retinal ganglion cells(RGC).The TUNEL staining was used to detect the apoptosis of retinal cells.The catalase(CAT),malondialdehyde(MDA),and superoxide dismutase(SOD)were measured to reflect the oxidative stress state of rats’retinas.Western blot was used to analyze the expression of NLRP3,ASC,and Caspase-1 proteins in the retina of all rats.Results The total retinal thickness of rats in the I/R group,I/R+GEN group,and sham operation group were(114.37±7.32)μm,(155.31±6.83)μm,and(178.98±13.65)μm,respectively.The differences among the three groups were statistically significant(t=14.284,all P<0.01).The thickness of IPL,INL,and GCL of rats were(18.95±5.06)μm,(17.62±4.69)μm,and(19.03±4.74)μm in the I/R group,(20.69±8.13)μm,(25.74±6.78)μm,and(26.71±7.85)μm in the I/R+GEN group,(11.73±3.15)μm,(11.97±3.56)μm,and(12.59±3.24)μm in the sham operation group(all P<0.05).The differences among the three groups were statistically s
作者
张静
ZHANG Jing(Department of Ophthalmology,the Second Affiliated Hospital of North Sichuan Medical College,Nanchong 637000,Sichuan Province,China)
出处
《眼科新进展》
CAS
北大核心
2021年第12期1122-1126,共5页
Recent Advances in Ophthalmology
基金
南充市科技局市校合作课题(编号19SXHZ0074)。
