摘要
【目的】观察解郁健脾补肾方对高催乳素血症的干预作用。【方法】将60只大鼠随机分为6组,即正常组,模型组,阳性药物组,中药低、中、高剂量组,每组10只。除正常组,其余各组大鼠采用皮下注射灭吐灵法构建高催乳素血症模型。成功造模后,阳性药物组给予溴隐亭2.0 mg·kg^(-1)·d^(-1)灌胃,中药低、中、高剂量组分别给予解郁健脾补肾方12.6、25.2、50.4 g·kg^(-1)·d^(-1)灌胃,正常组和模型组给予等体积蒸馏水灌胃,每日2次,共灌胃10 d。给药结束后处死大鼠,检测血清催乳素(PRL)、人促卵泡生成素(FSH)、雌二醇(E2)水平,采用蛋白免疫印迹(Western Blot)法检测卵巢组织转录信号转导子与激活子(STAT)5表达,荧光定量聚合酶链反应(PCR)法检测卵巢组织催乳素受体(PRLR)mRNA表达。【结果】与正常组比较,模型组血清PRL值明显升高,血清E2、FSH水平,STAT5蛋白、PRLR m RNA表达水平显著降低(P<0.05)。与模型组比较,各治疗组血清PRL值均明显降低,血清E2、FSH水平,STAT5蛋白、PRLR mRNA表达水平显著增高(P<0.05)。【结论】解郁健脾补肾方可有效降低高PRL水平,提高卵巢组织PRLR、STAT5表达,从而改善卵巢功能。
Objective To observe the intervention effect of Depression-Relieving, Spleen-Invigorating and Kidney-Tonifying Recipe on hyperprolactinemia in rats. Methods Sixty rats were randomly divided into 6 groups,namely normal group,model group,positive drug group,Chinese herbal medicine low-dose,medium-dose and high-dose groups, with 10 rats in each group. Except for the normal group, all the rats in other groups were injected subcutaneously with methotrexate to construct a hyperprolactinemia model. After successful modeling,the positive drug group was given 2.0 mg·kg^(-1)·d^(-1) of bromocriptine by gavage,the Chinese herbal medicine low-,medium-and high-dose groups were given 12.6,25.2 and 50.4 g·kg^(-1)·d^(-1) of Depression-Relieving,SpleenInvigorating and Kidney-Tonifying Recipe by gavage,respectively,and the normal group and model group were given equal volume of distilled water by gavage, all the treatment placed twice a day for 10 days. After the treatment, the rats were sacrificed to detect the levels of serum prolactin(PRL), human follicle stimulating hormone(FSH)and estradiol(E2),the expression of signal transducers and activators of transcription(STAT)5 in ovarian tissue was detected by Western Blot,mRNA expression of prolactin receptor(PRLR)in ovarian tissue was detected by fluorescence quantitative polymerase chain reaction(PCR). Results Compared with the normal group,the serum PRL value in the model group was significantly increased,and the serum E2,FSH levels,expression levels of STAT5 protein and PRLR m RNA were significantly decreased(P < 0.05). Compared with the model group,the serum PRL value in various treatment groups was significantly decreased,and the serum E2,FSH levels,expression levels of STAT5 protein and PRLR m RNA were significantly increased(P < 0.05). Conclusion Depression-Relieving, Spleen-Invigorating and Kidney-Tonifying Recipe is effective for reducing high PRL levele and increasing PRLR and STAT5 expression in ovarian tissues,so as to improve ovarian function.
作者
邓芳
高修安
许丽绵
DENG Fang;GAO Xiu-An;XU Li-Mian(Foshan Women and Children Hospital,Foshan 528000 Guangdong,China;The First Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510405 Guangdong,China)
出处
《广州中医药大学学报》
CAS
2021年第11期2474-2478,共5页
Journal of Guangzhou University of Traditional Chinese Medicine
基金
广东省中医药局科研项目(编号:20201372)。
关键词
解郁健脾补肾方
高催乳素血症
催乳素受体(PRLR)
信号转导子与激活子5(STAT5)
大鼠
Depression-Relieving
Spleen-Invigorating and Kidney-Tonifying Recipe
hyperprolactinemia
prolactin receptor(PRLR)
signal transducers and activators of transcription 5(STAT5)
rats
