摘要
蒙古岩黄芪是中国西北地区荒漠化修复和治理的主要灌木之一。随着土壤荒漠化、盐渍化的不断加剧,运用RNA-seq技术揭示荒漠植物的抗逆机制显得尤为重要。本研究通过转录组组装、质控并构建100 mmol/L NaCl胁迫下蒙古岩黄芪0、6和168 h转录组数据,利用BLAST软件在NR、SwissProt、COG、KEGG、GO和Pfam数据库中分别注释了45272、29738、36766、17143、39028和29640条Unigene;结果表明,蒙古岩黄芪基因的富集程度随盐胁迫时间的增加而增加;胁迫前期色氨酸代谢是主要代谢通路,而长期胁迫后增加了淀粉和蔗糖代谢相关的DEGs数量;且胁迫前期通过细胞色素、离子转运、清除活性氧等DEGs上调表达,后期通过积累渗透调节物质以适应盐环境。本研究为进一步挖掘和利用该植物抗逆基因资源提供了科学依据。
Hedysarum mongolicum is one of most the important shrubs for desertification restoration and government in Northwest China.With the aggravation of soil desertification and salinization,it is particularly important to reveal the resistance mechanism of desert plants by RNA-seq technology.In this study,the transcriptome data of H.mongolicum under 100 mmol/L Na Cl for 0,6 and 168 h were constructed by transcriptome assembly and quality control.45272,29738,36766,17143,39028 and 29640 unigenes were annotated by BLAST in NR,SwissProt,COG,KEGG,GO and PFAM databases,respectively.The results showed that with the extension of salt stress time,the gene enrichment degree of H.mongolicum increased gradually.Tryptophan metabolism was the main metabolic pathway in the early stage of stress,and the number of DEGs related to starch and sucrose metabolism was increased after long-term stress.In the early stage of stress,the expression of DEGs such as cytochrome,ion transport,and reactive oxygen species scavenging was up-regulation.In the late stage of stress,the osmotic adjustment substances accumulated to adapt to the salt environment.These results provide a scientific basis for the further development and utilization of stress-resistance gene resources of H.mongolicum.
作者
胡金鹏
潘雅清
张亚琪
周新雅
王强
覃滢卜
康鹏
Hu Jinpeng;Pan Yaqing;Zhang Yaqi;Zhou Xinya;Wang Qiang;Qin Yingbo;Kang Peng(College of Biological Science&Engineering,North Minzu University,Yinchuan,750021;Key Laboratory of Ecological Protection of Agro-pastoral Ecotones in the Yellow River Basin,National Ethnic Affairs Commission of the People's Republic of China,North Minza University,Yinchuan,750021;School of Agriculture,Ningxia University,Yinchuan,750021)
出处
《分子植物育种》
CAS
北大核心
2021年第23期7758-7770,共13页
Molecular Plant Breeding
基金
国家大学生创新创业训练计划项目(S2020-11407-013G)
北方民族大学科研启动资助项目(2019KYQD30)
北方民族大学高层次人才项目(2019BJLZ02)共同资助
宁夏回族自治区重点研发计划资助项目(2019BEB04018)。
关键词
蒙古岩黄芪
盐胁迫
转录组
基因注释
Hedysarum mongolicum
Salt stress
Transcriptome
Gene annotation
