摘要
目的筛选一种适合红曲茯苓片中多糖的含量测定方法。方法采用水提醇沉法提取红曲茯苓片中的多糖,分别采用苯酚硫酸法、蒽酮硫酸法和DNS法测定多糖含量,对比不同方法得到数据之间的差异和准确性。结果苯酚硫酸法和蒽酮硫酸法分别在0.01~0.12 mg和0.04~0.14 mg质量范围内线性良好,DNS法在0.02~0.06 mg/mL浓度范围内线性良好;苯酚硫酸法测定样品多糖稳定性、重复性和加样回收率均优于其他方法;含量测定结果分别为7.350 g/100g、8.975 g/100g和8.664 g/100g,蒽酮硫酸法测得值最高。相比而言,苯酚硫酸法显色稳定,重现性好,回收率高,结果更合理可靠。结论红曲茯苓片多糖含量测定时可优先考虑苯酚硫酸法。
Objective To select a suitable method for the determination of polysaccharides in Hongqu Fuling Tablets.Methods The polysaccharides in Hongqu Fuling Tablets were extracted by water extraction and alcohol precipitation method. The content of polysaccharides was determined by phenol sulfuric acid method, anthrone sulfuric acid method and DNS method, respectively. The differences and accuracy of data obtained by different methods were compared Results The linear values of phenol sulfuric acid method and anthrone sulfuric acid method were in the range of 0.01~0.12 mg and 0.04~0.14 mg, respectively. The linear values of DNS method were in the range of 0.02 ~ 0.06 mg/mL. The stability, reproducibility and recovery rate of the sample polysaccharides determined by phenol sulfuric acid method were better than other methods. The content determination results were 7.350 g/100 g, 8.975 g/100 g and 8.664 g/100 g, respectively, and the highest value was obtained by anthrone sulfuric acid method. Conclusion Content determination of polysaccharide in Hongqu Fuling Tablets should give priority to phenol sulfuric acid method.
作者
方杰
王成祥
王莉婷
石文筠
郭宇瑛
李颜伶
秦铭
马群
FANG Jie;WANG Cheng-xiang;WANG Li-ting;SHI Wen-jun;GUO Yu-ying;LI Yan-ling;QIN Ming;MA Qun(School of Chinese Medicine,Beijing University of Chinese Medicine,Beijing 102488,China)
出处
《时珍国医国药》
CAS
CSCD
北大核心
2021年第11期2672-2676,共5页
Lishizhen Medicine and Materia Medica Research
基金
国家重点研发计划中医药现代化研究重点专项(2018YFC1706800)。
关键词
红曲茯苓片
多糖
含量测定
苯酚硫酸法
蒽酮硫酸法
DNS法
Hongqu Fuling tablets
Polysaccharides
Content determination
Phenol sulfuric acid method
Anthrone sulfuric acid method
DNS method
