摘要
该文提出了实验教学改革的具体方法,并设计了超高效液相色谱/四极杆飞行时间质谱法测定地表水中磺胺甲恶唑含量的实验。通过实验,讲授了仪器的构造和原理、专业术语、操作步骤、数据分析,并自主设计实验,考察了正/负离子采集模式、有机相种类、传输区电压等对实验结果的影响,实现了实际水样中目标化合物浓度的检测,其中列举的常见故障及注意事项帮助学生顺利完成实验。该实验使学生熟悉了仪器的结构,原理及操作步骤,学习了化合物定性定量的基本方法以及主要参数的优化方法,提高了学生综合素质和创新能力,为今后的科研工作打下坚实的基础。
This paper develops a specific method for experimental teaching reform and designs an experiment using ultra performance liquid chromatography-mass spectrometry to determine the concentration of sulfamethoxazole in groundwater.During the experiments,the instrument’s construction and principles,technical terms,operation procedures,and data analysis have been introduced.Besides,to successfully quantify the target compounds in the aqueous samples,the effects of the ion acquisition modes,the types of the organic phases,and the voltage in the transmission zone on the determination results have been investigated with self-designed experiments.In addition,some common errors and notices have been introduced to help the students successfully finish the experiments.Therefore,after the experiment courses,students have learned the construction,principles,and operations of the instrument,the primary qualitative and quantitative methods for compounds,and optimization methods of major parameters,and improved their comprehensive ability and innovation skills,laying a solid foundation for the future researches.
作者
杨志敏
李四中
YANG Zhimin;LI Sizhong(Instrument Analysis Center,Huaqiao University,Xiamen 361021,China;College of Material Science and Engineering,Huaqiao University,Xiamen 361021,China)
出处
《实验科学与技术》
2021年第6期118-122,共5页
Experiment Science and Technology
基金
福建省教育厅中青年教师教育科研项目(JAT160031)
福建省石墨烯粉体及复合材料工程技术研究中心建设项目(2017H2001)
华侨大学国家自然科学培育基金(JBZR1214)。
