摘要
【目的】探究螯合剂[乙二胺二琥珀酸(EDDS)、氨三乙酸(NTA)和乙二醇二乙醚二胺四乙酸(EGTA)]对青葙镉(Cd)、铅(Pb)、锌(Zn)和铜(Cu)吸收累积的影响,为螯合剂和青葙在环境修复中的应用提供数据支撑。【方法】采用大棚内盆栽种植青葙试验方法,以不添加螯合剂作对照(CK),研究螯合剂EDDS(1.0、2.0和3.0 mmol/kg)、NTA(1.0、2.0和3.0 mmol/kg)和EGTA(1.0、2.0和3.0 mmol/kg)作用下青葙对土壤Cd、Pb、Zn和Cu的吸收与转运。【结果】与CK相比,1.0和2.0 mmol/kg EDDS、1.0~3.0 mmol/kg NTA、1.0和2.0 mmol/kg EGTA能显著促进青葙生长(P<0.05,下同),其中1.0 mmol/kg EGTA促进效果最大,其干重较CK增加37.5%。1.0和2.0 mmol/kg EDDS、2.0和3.0 mmol/kg NTA、1.0和2.0 mmol/kg EGTA能显著促进青葙对Cd的吸收累积,且2.0 mmol/kg EGTA作用下,青葙中Cd的生物富集系数和转运系数提升最大,分别是CK的1.55和1.61倍;2.0和3.0 mmol/kg EDDS、1.0~3.0 mmol/kg NTA及1.0~3.0mmol/kg EGTA均能显著促进青葙对Pb的吸收累积,且Pb在青葙中的生物富集系数和转运系数较CK均有明显提升,以3.0 mmol/kg EGTA处理的青葙地上部富集系数和转移系数最大,分别是CK的1.63和1.47倍;1.0和2.0 mmol/kg EDDS、2.0 mmol/kg EGTA能显著促进青葙对Zn的吸收累积,以1.0 mmol/kg EDDS处理的富集系数最大,是CK的1.18倍,2.0 mmol/kg NTA处理的转移系数最大,是CK的1.22倍;1.0~3.0 mmol/kg EDDS、1.0~3.0 mmol/kg NTA和1.0~3.0 mmol/kg EGTA均能显著促进青葙对Cu的吸收累积,以2.0 mmol/kg EDDS处理的青葙地上部富集系数最大,是CK的2.07倍,3.0 mmol/kg EGTA处理的青葙地上部转移系数最大,是CK的2.52倍。【结论】在本研究螯合剂施用剂量范围内,除3.0 mmol/kg EGTA减少青葙吸收累积Cd和3.0 mmol/kg EDDS、3.0 mmol/kg NTA、1.0 mmol/kg EGTA及3.0 mmol/kg EGTA减少青葙吸收累积Zn外,3种螯合剂的其余剂量均可促进青葙对Cd、Pb、Zn和Cu的吸收富集。因此,适当剂量的螯合剂作为诱�
【Objective】The experimental objective was to investigate the effects of chelators[ethylene diamine disuccinate(EDDS),nitrilotriacetic acid(NTA)and glycol-bis-(2-aminoethylether)-N,N,N ′,N ′-tetraacetic acid(EGTA)]on the uptake and accumulation of cadmium(Cd),lead(Pb),zinc(Zn)and copper(Cu)with Celosia argentea L.,and to provide data support for the application of chelators and C.argentea L.in environmental repairation.【Method】The application effects of EDDS(1.0,2.0 and 3.0 mmol/kg),NTA(1.0,2.0 and 3.0 mmol/kg)and EGTA(1.0,2.0 and 3.0 mmol/kg)were evaluated on the uptake and transportation of heavy metals(Cd,Pb,Zn and Cu)from soil with C.argentea L.by pot experiment in greenhouse,and without the addition of chelating agent as contrast(CK).【Result】The results indicated that1.0-2.0 mmol/kg EDDS,1.0-3.0 mmol/kg NTA,1.0-2.0 mmol/kg EGTA promoted significantly the growth of C.argentea L.(P<0.05,the same below)compared with CK,and among them,1.0 mmol/kg EGTA had the best effect,the dry weight increased by 37.5% than that of CK.1.0-2.0 mmol/kg EDDS,2.0-3.0 mmol/kg NTA,1.0-2.0 mmol/kg EGTA significantly promoted the absorption and accumulation of Cd in C.argentea L.,and the highest bioconcentration factors and translocation factor were respectively 1.55 and 1.61 times as those of CK with applying 2.0 mmol/kg EGTA.2.0-3.0 mmol/kg EDDS,1.0-3.0 mmol/kg NTA and 1.0-3.0 mmol/kg EGTA could significantly promote the absorption and accumulation of Pb in C.argentea L.,and the bioconcentration factors and translocation factor of Pb in C.argentea L.were improved compared with CK,and the highest bioconcentration factors and translocation factor were respectively 1.63 and 1.47 times as those of CK with applying 3.0 mmol/kg EGTA.1.0 and 2.0 mmol/kg EDDS and 2.0 mmol/kg EGTA could significantly promote the absorption and accumulation of Zn in C.argentea L.,and the highest bioconcentration factor was as 1.18 times as that of CK when applying 1.0 mmol/kg EDDS.With applying 2.0 mmol/kg NTA,and the highest translocation fact
作者
陈伟
廖洁
杨玉霞
蒋文艳
王海军
李晓妤
莫磊兴
王天顺
CHEN Wei;LIAO Jie;YANG Yu-xia;JIANG Wen-yan;WANG Hai-jun;LI Xiao-yu;MO Lei-xing;WANG Tian-shun(Research Institute of Agro-products Quality Safety and Testing Technology,Guangxi Academy of Agriculture Sciences/Quality Supervision and Testing Center for Sugarcane,Ministry of Agriculture and Rural Affairs,Nanning 530007,China)
出处
《南方农业学报》
CAS
CSCD
北大核心
2021年第9期2447-2456,共10页
Journal of Southern Agriculture
基金
广西自然科学基金面上项目(2020GXNSFAA259051)
广西农业科学院基本科研业务专项(桂农科2021YT137)
广西农业科学院科技先锋队专项(桂农科JZ202020)。
关键词
螯合剂
青葙
重金属
土壤
chelator
Celosia argentea L.
heavy metals
soil
