摘要
目的:探讨血清淀粉样蛋白A(SAA)、人附睾蛋白4(HE4)与血清糖类抗原125(CA125)联合检测在卵巢肿瘤早期诊断的临床意义,以提高卵巢癌早期诊断率。方法:选取2019年5月~2020年5月在我院就诊的60例卵巢肿瘤患者为研究对象,其均经活检确诊,其中恶性卵巢肿瘤患者30例,作为研究组,良性卵巢肿瘤患者30例,作为常规组,同时选择30例健康受试者作为对照组。分别在罗氏CobasE602全自动免疫化学发光分析仪中利用电化学发光法及配套试剂测定血清中HE4及CA125的水平;在Ottman-1000特定蛋白分析仪中利用乳胶增强免疫比浊法及配套试剂测定全血中SAA的水平。结果:研究组患者HE4、CA125及SAA的水平明显高于常规组和对照组,差异具有统计学意义(P<0.05),而常规组和对照组之间差异则无统计学意义(P>0.05);两组卵巢肿瘤患者HE4+CA125+SAA联合诊断的阴性预测值,漏诊率,灵敏度和特异度均好于HE4与CA125单独检测,且差异具有统计学意义(P<0.05)。结论:SAA、HE4及CA125联合检测对于诊断卵巢癌具有简便、快速、成本低、无创伤性等优势,显著提高检测的灵敏度和特异性,有助于女性卵巢肿瘤的早发现,早治疗,提高患者生存率。
Objective:To probe into the clinical significance of combined detection of serum amyloid A(SAA),human epididymal protein 4(HE4)and serum carbohydrate antigen 125(CA125)in early diagnosis of ovarian tumors,in order to improve the early diagnosis rate of ovarian cancer.Methods:60 cases of patients with ovarian cancer and who treated in our hospital from May 2019 to May 2020 were selected as the research objects,and the patients were confirmed by biopsy,including 30 cases of malignant ovarian tumor as the study group,30 cases of benign ovarian tumor as the routine group,and 30 cases of healthy subjects in the same period as the control group.Then,the levels of HE4 and CA125 in serum were measured by electrochemiluminescence and matching reagents in the Roche cobase602 automatic immunochemiluminescence analyzer,and the SAA in whole blood were determined by latex enhanced immunoturbidimetry and matching reagents in ottman-1000 protein analyzer.Results:The levels of HE4,CA125 and SAA in the study group were significantly higher than those in the control group and the control group,and the difference was statistically significant(P<0.05).There was no significant difference between the routine group and the control group(P>0.05);The negative predictive value,missed diagnosis rate,sensitivity and specificity of the combined diagnosis of serum HE4+CA125+SAA of two groups were better than those in the single detection of HE4 and CA125,and the difference was statistically significant(P<0.05).Conclusion:The combined detection of SAA,HE4 and CA125 has the advantages of simplicity,rapidity,low cost and noninvasive for the diagnosis of ovarian cancer,which can significantly improve the sensitivity and specificity of detection,and it is help the early detection,early treatment and improve the survival rate of women with ovarian cancer.
作者
李乔
吴健
钟志辉
LI Qiao;WU Jian;ZHONG Zhi-hui(Department of laboratory,Gaozhou people's Hospital,Guangdong Province,Guangdong Gaozhou 525200)
出处
《医学检验与临床》
2021年第11期1-3,73,共4页
Medical Laboratory Science and Clinics
基金
茂名市科技计划项目(2019007)。
