摘要
目的通过观察保元汤对秀丽隐杆线虫的影响,探讨保元汤的抗衰老作用及机制。方法采用野生型N2秀丽隐杆线虫,进行同步化处理后分为空白对照组、溶剂对照组、保元汤组,空白对照组不加任何药物,溶剂对照组加二甲基亚矾溶剂,保元汤组分别加1000、500、200、100、50μg/mL的保元汤提取物药液培养,观察对秀丽隐杆线虫寿命、生殖力、运动能力、头部摆动及热应激、氧化应激损伤的影响。结果溶剂对照组秀丽隐杆线虫平均寿命与空白对照组比较差异无统计学意义(P>0.05),而保元汤100、200、500μg/mL组秀丽隐杆线虫平均寿命较空白对照组均明显延长(P<0.05)。溶剂对照组及保元汤各组秀丽隐杆线虫平均产卵量与空白对照组比较差异均无统计学意义(P>0.05)。溶剂对照组秀丽隐杆线虫1 min平均运动峰数、1 min平均摆动次数与空白对照组比较差异无统计学意义(P>0.05),而保元汤200、500、1000μg/mL组秀丽隐杆线虫1 min平均运动峰数、1 min平均摆动次数较空白对照组均明显增多(P<0.05)。热应激测试后第3天空白对照组和溶剂对照组秀丽隐杆线虫几乎全部死亡,而保元汤200、500、1000μg/mL组第1、2、3天存活率均明显高于空白对照组(P<0.05)。氧化应激测试第4天空白对照组和溶剂对照组秀丽隐杆线虫几乎全部死亡,而保元汤200、500、1000μg/mL组第1、2、3、4天存活率均明显高于空白对照组(P<0.05)。结论保元汤能够有效延长秀丽隐杆线虫的平均寿命,而并不影响其生殖功能,具有延缓衰老的作用,其机制可能与提高秀丽隐杆线虫抗应激能力有关。
Objective To observe the effect of Baoyuan Decoction(BY)for Caenorhabditis elegans(C.elegans)to discuss anti-aging mechanism.Methods C.elegans,wild-type(WT)N2 strain,were divided into blank group,solvent group and BY groups after synchronization treatment.Blank group was cultured without drugs,solvent group with DMSO,and BY groups with different doses extract solution(1000,500,200,100,50μg/mL),respectively.The changes of lifespan,fecundity,exercise capacity,head sway,heat stress and oxidative stress due to intervention were observed.Results Difference was significant in average lifespan between solvent group and blank group(P>0.05),but average lifespan in BY groups(100,200,500μg/mL)was significantly increased(P<0.05);no difference was significant in average fecundity between solvent group and blank group,and between BY groups and blank group(P>0.05),and no difference in average number of 1-min peak value,1-min swing times between blank group and solvent group(P>0.05);but BY groups(200,500,1000μg/mL)was significantly increased(P<0.05).Through heat stress testing on the 3rd day and oxidative stress testing on the 4th day,the Results showed that almost all the strain in blank group and solvent group were death,survival rate on the 1th,2nd,3rd 4th day was significantly higher in BY groups(200,500,1000μg/mL)than in blank control group(all P<0.05).Conclusion For C.elegans,BY can effectively prolong the average lifespan and does not affect its reproductive function,has anti-aging effect.The mechanism may be related to the anti-stress ability.
作者
张训乐
王姗姗
赵佳惠
黄家情
闻妩
贡岳松
ZHANG Xunle;WANG Shanshan;ZHAO Jiahui;HUANG Jiaqing;WEN Wu;GONG Yuesong(Nanjing University of Chinese Medicine,Jiangsu,Nanjing 210046;不详)
出处
《河北中医》
2021年第9期1519-1524,共6页
Hebei Journal of Traditional Chinese Medicine
关键词
衰老
保元汤
实验研究
线虫
Aging
Baoyuan Decoction
Experimental study
Caenorhabditis elegans
