摘要
防御酶是植物对昆虫危害的潜在防御机制。毛花雀稗(Paspalum dilatatum)作为一种优良牧草,了解被昆虫取食胁迫后其防御酶的变化很有必要。为此,本研究分析了蝗虫(Oxya chinensis)取食毛花雀稗0(CK)、24、48、72和96 h后毛花雀稗总抗氧化能力(total antioxidant capacity,T-AOC)、过氧化物酶(peroxidase,POD)、过氧化氢酶(catalase,CAT)、超氧化物歧化酶(superoxide dismutase,SOD)、酪氨酸解氨酶(tyrosine ammonia-lyase,TAL)、多酚氧化酶(polyphenol oxidase,PPO)、脂氧合酶(lipoxygenase,LOX)、谷胱甘肽S-转移酶(glutathione S-transferase,GST)、苯丙氨酸解氨酶(phenylalanine ammonia-lyase,PAL)活性及植物总酚(total phenols,TP)含量的变化规律。结果表明:与不取食对照相比,蝗虫取食后毛花雀稗植株中POD、CAT、LOX、PPO、TAL、GST活性以及TP含量均在部分取食时间或所有取食时间显著升高(P<0.05)。其中T-AOC在24 h时显著升高(P<0.05);TAL、PPO和GST活性均随取食时间的延长呈先升高后降低的变化趋势,分别在48、72和24 h时达到最高,是对照的3.71、2.10和21.25倍。相反,除24 h时SOD活性外,SOD、PAL活性在蝗虫取食胁迫下均显著降低(P<0.05)。可见,POD、CAT、PPO、TAL、GST和LOX等防御酶在毛花雀稗防御害虫胁迫中发挥了非常重要的保护作用。
Defense enzymes are potential defense mechanisms against herbivorous insects in plants.It is important to learn about the changes in protective enzymes in response to insect-induced stresses in excellent forages,such as dallisgrass(Paspalum dilatatum).In the present study,total antioxidant capacity(T-AOC),peroxidase(POD),catalase(CAT),superoxide dismutase(SOD),tyrosine ammonia-lyase(TAL),lipoxygenase(LOX),polyphenol oxidase(PPO),glutathione S-transferase(GST),and phenylalanine ammonia-lyase(PAL),as well as total phenol(TP)content of dallisgrass were investigated at 0,24,48,72,and 96 h after grasshopper(Oxya chinensis)feeding.The results showed that the activities of POD,LOX,CAT,PPO,TAL,and GST as well as the TP content in dallisgrass were significantly increased after grasshopper feeding compared with the control(P<0.05).T-AOC increased significantly 24 h after feeding(P<0.05).Moreover,the activities of PPO,TAL,and GST first increased and then decreased over time.At 48,72,and 24 h of treatment,the activity of TAL,PPO,and GST peaked at 3.71,2.10 and 21.25 times of CK,respectively.In contrast,grasshopper feeding significantly reduced the activity of SOD and PAL(P<0.05).Overall,it could be speculated that protective enzymes such as POD,CAT,PPO,TAL,GST,and LOX play very important protective roles against insect herbivores.
作者
何应
马向丽
任健
代微然
毛如志
HE Ying;MA Xiangli;REN Jian;DAI Weiran;MAO Ruzhi(Faculty of Animal Science and Technology,Yunnan Agricultural University,Kunming 650201,Yunnan,China;Division of Science and Technology,Yunnan Agricultural University,Kunming 650201,Yunnan,China;College of Food Science and Technology,Yunnan Agricultural University,Kunming 650201,Yunnan,China)
出处
《草业科学》
CAS
CSCD
北大核心
2021年第11期2294-2300,共7页
Pratacultural Science
基金
云南省重点研发计划项目子课题(2018BB001)。
关键词
蝗虫
毛花雀稗
总抗氧化能力
超氧化物歧化酶
植物总酚
多酚氧化酶
过氧化物酶
grasshopper
Paspalum dilatatum
total antioxidant capacity
superoxide dismutase
total phenol
polyphenol oxidase
peroxidase
