摘要
针对皖西大别山金寨县永福桑黄菌种植合作社种植的桑黄菌开展研究,通过现代分子生物学技术对其18S rDNA基因ITS区段进行克隆测序和序列特征分析,依据ITS序列分析结果将菌种鉴定为瓦宁木层孔菌(Sanghuangporus vaninii);对JS-1的基础培养基进行筛选,结果显示,PDA培养基最适合其生长;测定了JS-1菌株的生长特性,结果显示,温度在5~35℃区间内菌丝均可生长,最适生长温度为28℃;pH在4~10范围内菌丝均可生长,pH为7时最适合菌丝生长。以菌丝生长情况为指标筛选碳源和氮源,碳源为葡萄糖的培养基上菌丝生长速度最快且长势浓密,认为葡萄糖为其生长最适碳源;以马铃薯浸汁为氮源的培养基虽然生长速度略慢于蛋白胨和玉米粉,但菌落长势最为茂盛,且最早出现黄褐色软木状环纹,认为马铃薯浸汁为其生长最适氮源;在以葡萄糖为碳源,马铃薯浸汁为氮源的基础上,通过L_(9)(3^(4))正交试验得到JS-1菌株菌丝生长速度最快的培养条件为葡萄糖20 g·L^(-1),马铃薯100 g·L^(-1),pH为8,培养温度30℃;测定菌丝的致死温度为45℃。
In this study,researchers conducted a research on the strains of Sanghuang planted by Yongfu Plantation Cooperation of Jinzhai Country in Dabie Mountain Area and identified the strain as Sanghuang vaninii by cloning and sequencing the ITS region of its 18S rDNA gene using modern molecular biology techniques.Screening of the basal medium for JS-1 showed that PDA medium was the most suitable for the growth of JS-1.The growth characteristics of JS-1 strain were determined,and the results showed that the mycelium could grow in the temperature range of 5-35℃,and the optimum growth temperature was 28℃.The mycelium could grow in the pH range of 4-10,while the pH value of 7 was the optimal for the growth of mycelium.By taking the mycelium growth as an indicator to screen the carbon and nitrogen sources,it was found that the medium had the fastest and dense mycelium growth when glucose was used as the carbon source.Thus,glucose was considered the most suitable carbon source.When potato juice was used as the nitrogen source of the medium,despite of the slightly slower growth rate than peptone and corn flour,the colony growth was the most luxuriant,with the earliest appearance of yellow-brown cork-like ring.Thus,potato juice was considered the most suitable nitrogen source for its growth.On the basis of glucose as carbon source and potato extract as nitrogen source,L_(9)(3^(4))orthogonal test showed that the optimal growth conditions of JS-1 strain were glucose 20 g·L^(-1) and potato 100 g·L^(-1),pH 8 and culture temperature 30℃.The mycelium lethal temperature was 45℃.
作者
程安东
汪本勤
CHENG Andong;WANG Benqin(Lu'an Vocational Technical College, Lu'an 237158, China)
出处
《浙江农业学报》
CSCD
北大核心
2021年第12期2234-2244,共11页
Acta Agriculturae Zhejiangensis
基金
安徽省教育厅自然科学重点研究项目(KJ2019A1062)。
关键词
桑黄
菌种鉴定
培养基
生长条件
Sanghuang
species identification
medium
growth conditions
