摘要
目的检测RAD51、XRCC4蛋白在食管鳞癌细胞系TE-1和正常食管上皮细胞株HEEp及有淋巴结转移的食管鳞癌组织中的表达情况,讨论RAD51、XRCC4对食管鳞癌发生、发展及预后的影响。方法收集川北医学院附属医院病理科2017~2018年食管癌根治术标本62例。免疫细胞化学方法检测RAD51、XRCC4蛋白在食管鳞癌细胞系TE-1细胞和正常食管上皮细胞株HEEp中的表达;免疫组织化学法检测RAD51、XRCC4蛋白在62例有淋巴结转移的食管鳞癌组织中的表达;运用Kaplan-meier分析RAD51、XRCC4蛋白阳性表达及阴性表达患者的无病进展生存期PFS。结果免疫细胞化学方法显示RAD51、XRCC4蛋白在食管癌细胞系TE-1细胞中呈阳性表达,而正常食管上皮细胞株HEEp细胞中呈阴性表达;免疫组织化学法显示RAD51、XRCC4蛋白在食管鳞癌组织中表达上调,与癌旁组织及配对癌转移淋巴结相比差异具有统计学意义(P<0.05),除了RAD51蛋白阳性表达率在女性中高于男性(P<0.05),两者的阳性表达率在其他临床病理特征如肿瘤直径、浸润深度、分化程度、P-TNM分期中均差异无统计学意义(均P>0.05)。RAD51、XRCC4阳性表达患者与阴性表达患者的PFS比较差异无统计学意义(均P>0.05)。结论RAD51、XRCC4可能通过参与DNA双链断裂修复,从而参与了食管鳞癌的发生发展。
Objective To detect the expression of RAD51 and XRCC4 proteins in esophageal squamous cell line TE-1,normal esophageal epithelial cell line HEEp and esophageal squamous cell carcinoma with lymph node metastasis,and discuss the effects of RAD51 and XRCC4 on the occurrence,development and prognosis of esophageal squamous cell carcinoma.Methods The expression of RAD51 and XRCC4 proteins in TE-1 cells of esophageal squamous cell lines and HEEp of normal esophageal epithelial cells were detected by immunocytochemistry.Immunohistochemistry was used to detect the expression of RAD51 and XRCC4 proteins in 62 cases of esophageal squamous cell carcinoma with lymph node metastasis.Kaplan-meier was used to analyze the PFS of patients with positive and negative expression of RAD51 and XRCC4 protein.Results The immunocytochemical method showed that RAD51 and XRCC4 proteins were positively expressed in TE-1 cells of esophageal carcinoma cell line,and negatively expressed in HEEp cells of normal esophageal epithelial cell line.Immune histochemical method showed that RAD51,XRCC4 protein expression in esophageal squamous carcinoma tissue,compared with the tissue adjacent to carcinoma and paired cancer metastasis lymph nodes,the difference is statistically significant(P<0.05),in addition to RAD51 protein positive expression rate in women than men(P<0.05),and the positive expression rate in other clinical pathological features such as tumor diameter,infiltrating depth and differentiation degree,p-TNM stage had no obvious difference in values(P>0.05).There was no significant difference in PFS between patients with RAD51 and XRCC4 positive expression and patients with negative expression(all P values were>0.05).Conclusion RAD51 and XRCC4 may participate in the occurrence and development of esophageal squamous cell carcinoma by participating in DNA double-strand damage repair,but the specific mechanism remains to be further studied.
作者
张仁静
李雨珊
莫琳
蹇顺海
何欣蓉
ZHANG Renjing;LI Yushan;MO Lin;JIAN Shunhai;HE Xinrong(Department of Pathology,The Affiliated Hospital of North Sichuan Medical College,Nanchong 637000, Sichuan, China;Department of Pathology, North Sichuan Medical College, Nanchong 637000, Sichuan, China;Nanchong Central Hospital, Nanchong 637000, Sichuan, China)
出处
《西部医学》
2021年第12期1753-1758,共6页
Medical Journal of West China
基金
南充市科学技术局(19SXHZ0356)
南充市科学技术局(20SXQT0023)。
关键词
食管鳞癌
淋巴结转移
RAD51
XRCC4
Esophageal squamous cell carcinoma
Lymph node metastasis
RAD51
XRCC4
