摘要
目的探讨西北地区绝经后骨质疏松症(postmenopausal osteoporosis,PMOP)患者肠道微生物(gut microbiota,GM)组分和基因功能变化,及其与骨密度(bone mineral density,BMD)的相关性。方法以2018年11月—2019年10月到我院就诊的绝经后女性作为初筛对象,选取首次诊断为骨质疏松症(OP)患者24例、低骨量者30例及骨量正常者9例,收集粪便样本并提取微生物DNA,借助Illumina平台进行16S rRNA基因和宏基因组高通量测序分析。结果GM结构在不同组中存在差异,消化链球菌科、罗姆布茨菌属、梭菌目未确认属、巨单胞菌属、Erysipelatoclostridium、克雷伯氏菌属和Erysipelatoclostridium ramosum为OP组差异菌群。GM基因注释结果显示基因数目与BMD值呈正相关,基因KEGG功能注释结果显示新陈代谢、碳水化合物代谢、淀粉和蔗糖代谢、氧化磷酸化基因功能条目均与BMD值呈负相关。受试者工作特征曲线(receiver operating characteristic curve,ROC)分析提示,碳水化合物代谢、淀粉和蔗糖代谢、氨基糖和核苷酸糖代谢、氧化磷酸化对鉴别OP分别具有较好的灵敏度和特异度,ROC曲线下面积(area under curve,AUC)分别为0.70、0.72、0.73和0.75。结论高通量测序技术有助于分析PMOP患者GM组成、分布和基因功能改变,为研究GM调控BMD变化进而影响OP的发生发展提供依据。
Objective To explore the profile and gene functional changes of gut microbiota(GM)in women with postmenopausal osteoporosis(PMOP)in Northwest China,and the correlations between GM and bone mineral density(BMD).Methods From November 2018 to October 2019,postmenopausal women were screened on their initial visits to our hospital,and 24 new osteoporosis(OP)patients,30 new osteopenia patients and nine negative controls were recruited.Fecal samples were collected for GM DNA extraction,and Illumina platforms were used for high-throughput sequencing of 16S rRNA and metagenome.Species annotation,GM profile and gene functions were viewed and analyzed.Results GM profiles were significatly different in different groups,and the LDA scores of Peptostreptococcaceae,Romboutsia,unidentified Clostridiales,Megamonas,Erysipelatoclostridium,Klebsiella and Erysipelatoclostridium ramosum were more than 3 in OP group.Metagenomic sequencing analysis indicated that gene numbers were positively correlated with BMD,and metabolism,carbohydrate metabolism,starch and sucrose metabolism and oxidative phosphorylation were negatively correlated with BMD.Receiver operating characteristic curve(ROC)showed that carbohydrate metabolism,starch and sucrose metabolism,amino sugar and nucleotide sugar metabolism,oxidative phosphorylation,respectively,could identify OP with preferable sensitivity and specificity(areas under curve were 0.70,0.72,0.73 and 0.75,respectively).Conclusions High-throughput sequencing had great potential for GM analysis of postmenopausal women with OP,providing evidence of the correlations between GM and BMD.
作者
吕静
赵和平
于燕
曾玉红
代坤
乔蓉
郭磊
王冀邯
Lyu Jing;Zhao Heping;Yu Yan;Zeng Yuhong;Dai Kun;Qiao Rong;Guo Lei;Wang Jihan(Department of Clinical Laboratory,Honghui Hospital,Xi′an Jiaotong University,Xi′an 710054,China;Department of Osteoporosis,Honghui Hospital,Xi′an Jiaotong University,Xi′an 710054,China;Department of Clinical Laboratory,Yanliang Railway Hospital of Xi′an,Xi′an 710089,China;Department of Spinal Surgery,Honghui Hospital,Xi′an Jiaotong University,Xi′an 710054,China;Institute of Medical Research,Northwestern Polytechnical University,Xi′an 710072,China)
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2021年第11期867-874,共8页
Chinese Journal of Microbiology and Immunology
基金
陕西省自然科学基金(2019JQ-978)
西安市卫健委面上培育项目(2020ms14)
国家自然科学基金青年科学基金(81702067)。
关键词
绝经后骨质疏松症
骨密度
肠道微生物
宏基因组测序
Postmenopausal osteoporosis
Bone mineral density
Gut microbiota
Metagenomic sequencing
