摘要
背景:传统"二袖套法"大鼠肝移植模型的建立时间长,训练难度大,难以适应目前研究生的培养模式。目的:利用大鼠肝移植建立步骤多的特点,合理组合每一步骤的时间及方法,缩短学习时间,降低操作难度,快速地构建大鼠肝移植模型。方法:以"二袖套法"为基础,在保留下腔静脉血流通畅的基础上门静脉优先套管吻合,套管采用供肝放在大鼠下腹部的反向吻合法(门脉优先组),建立大鼠肝移植模型,与传统大鼠肝移植模型(传统移植组)予以对比,统计分析两组学习曲线时间、门静脉阻断时间、无肝期时间、肝下下腔静脉阻断时间、受体手术时间及术后生存情况等。结果与结论:(1)对传统移植组32只和门脉优先组30只模型建立的学习曲线进行分析,门脉优先组学习时间短于传统移植组;(2)将存活时间超过2 d的传统移植组12只大鼠和门脉优先组20只大鼠手术中的各阶段时间进行统计,采用参数检验分析显示,门脉优先组门静脉阻断时间稍长于传统移植组但差异无显著性意义;而门脉优先组肝上下腔阻断时间为(15.0±2.2)min,明显短于传统移植组的(21.3±2.1)min(P<0.05);门脉优先组肝下下腔阻断时间为(25.0±2.9)min,明显短于传统移植组的(32.5±3.2)min(P<0.05);两组供体灌注时间、供体手术时间、供体肝脏修整时间和受体手术时间相比差异无显著性意义;(3)提示门脉优先肝移植大鼠模型能缩短学习周期,降低操作难度,为初学者迅速掌握大鼠肝移植模型建立提供了一种较为可靠的方法,适合学习和推广。
BACKGROUND:The traditional“two cuff”model of rat liver transplantation takes a long time to establish,which is difficult to grasp and adapt to the training of graduate students.OBJECTIVE:As per the characteristics of multi-step establishment of liver transplantation model in rats,to rationally combine the time and method of each step so as to shorten the learning time and reduce the difficulty of operation,thereby rapidly establishing a rat model of liver transplantation.METHODS:The portal vein was preferentially anastomosed using the two-cuff method when the blood flow of the inferior vena cava was unobstructed.The cuff was anastomosed reversely by placing the donor liver in the lower abdomen of rats(portal vein preferential anastomosis group).The rat liver transplantation model was established and compared with the traditional rat liver transplantation model(traditional transplantation group).The learning time curve,portal vein occlusion time,anhepatic phase time,inferior vena cava occlusion time,recipient operation time,and postoperative survival of the two groups were statistically analyzed.RESULTS AND CONCLUSION:The learning curves of 32 cases in the traditional transplantation group and 30 cases in the portal vein preferential anastomosis group were analyzed.The learning time of portal vein preferential anastomosis group was shorter than that of traditional transplantation group.The operation time of 12 rats in the traditional transplantation group and 20 rats in the portal vein preferential anastomosis group which survived over 2 days was statistically analyzed.Parameter test analysis showed that the portal vein occlusion time in the portal vein preferential anastomosis group was slightly longer than that in the traditional transplantation group,but there was no significant difference.However,the blocking time of the suprahepatic inferior vena cava in the portal vein preferential anastomosis group was(15.0±2.2)minutes,which was significantly shorter than(21.3±2.1)minutes in the traditional transplant
作者
张继翔
闫宏宪
轩娟娟
白鸿太
王耀权
魏思东
陈国勇
Zhang Jixiang;Yan Hongxian;Xuan Juanjuan;Bai Hongtai;Wang Yaoquan;Wei Sidong;Chen Guoyong(Henan University People’s Hospital,Zhengzhou 450003,Henan Province,China;Luoyang Central Hospital,Zhengzhou University,Luoyang 471000,Henan Province,China)
出处
《中国组织工程研究》
CAS
北大核心
2022年第17期2696-2701,共6页
Chinese Journal of Tissue Engineering Research
基金
国家自然科学基金资助项目(U2004124),项目负责人:陈国勇
河南省医学科技攻关计划项目(SBGJ2018071),项目负责人:陈国勇
河南省医学科技攻关计划项目(SB201903020),项目负责人:魏思东。
关键词
门脉优先
肝移植
反向吻合
学习曲线
无肝期
portal vein preferential anastomosis
liver transplantation
reverse anastomosis
learning curve
anhepatic phase
