摘要
背景:肝移植供体缺乏,脂肪肝在人群中发病率高,中度以上脂肪肝作为边缘供体,肝移植后移植物丢失风险高,为提高这类供体利用率,需要建立稳定的大鼠模型作为研究对象。目的:建立适合脂肪肝供肝的稳定大鼠减体积肝移植模型。方法:随机各选取20对SD-SD脂肪肝供肝肝移植大鼠,全体积组行全体积原位肝移植术,减体积组行减体积肝移植术。供肝恢复灌流后,观察记录两组大鼠呼吸动度、呼吸频率、心跳及大血管充盈情况,术后观察大鼠恢复情况,解剖死亡大鼠了解死因;记录两组手术时间并作比较;两组大鼠术后进行生存分析;比较两组大鼠术后肝功能及病理组织学改变,评估差异性。结果与结论:(1)供肝恢复灌注后,全体积组肝移植受体大鼠呼吸频率、心率快于减体积肝移植组受体大鼠(P<0.05),下腔静脉充盈情况相对较差;(2)减体积组受体大鼠术后一般情况恢复好于全体积组大鼠;(3)全体积组修肝时间短于减体积组,但受体手术时间明显长于减体积组(P<0.05);全体积组受体大鼠术后早期死亡率明显高于减体积组,主要死亡原因是腹腔出血、空气栓塞和低血容量性休克;两组受体大鼠后续存活时间比较差异无显著性意义;(4)两组受体大鼠术后肝功能肝酶学和总胆红素总体差异无显著性意义,病理组织学表现相似;(5)综上,脂肪肝供肝大鼠减体积肝移植模型稳定可靠,解决了脂肪肝供肝全体积肝移植术中术野暴露不清、术后易致受体大鼠循环失稳的问题,是研究脂肪肝供肝肝移植的理想动物模型之一。
BACKGROUND:Because of liver donor deficiency and high incidence of fatty liver,moderate fatty liver as a marginal donor results in a high risk for graft loss after liver transplantation.Therefore,it is imperative to establish a stable rat model by which we can enhance the utilization of these liver donors.OBJECTIVE:To establish a viable and stable model of reduced-size liver transplantation with fatty liver donor in rats.METHODS:Forty pairs of fatty liver donor and recipient rats were randomly chosen.The recipient rats in experimental group received reduced-size liver transplantation and the rats in control group received orthotopic liver transplantation.The breath,heart rate,and vessel filling situation were observed.The postoperative recovery,cause of death,and survival conditions were observed.The operation time was compared between two groups.Besides,the postoperative liver function and pathological changes were assessed.RESULTS AND CONCLUSION:After graft reperfusion,in the control group,the respiratory rate and heart rate were significantly faster than those in experimental group(P<0.05),but the vessel filling of inferior vena cava was poor.Rats in the experimental group recovered better than those in the control group(P<0.05).The operation time in the control group was significantly prolonged compared with the experimental group(P<0.05).The early mortality in the control group was significantly higher than that in the experimental group,which mainly caused by intra-abdominal hemorrhage,air embolism and hypovolemic shock.The survival time,histology and liver function indexes postoperatively did not differ significantly between two groups.To conclude,the rat model of reduced-size liver transplantation is stable,which reveals clear field of operation and stable circulation,so it is a ideal model for liver transplantation with fatty liver donors.
作者
赵英鹏
李立
陈刚
白建华
刘其雨
Zhao Ying-peng;Li Li;Chen Gang;Bai Jian-hua;Liu Qi-yu(Department of Hepatobiliary and Transplantation Surgery,the First Hospital of Kunming&Affiliated Ganmei Hospital of Kunming Medical University,Kunming 650011,Yunnan Province,China)
出处
《中国组织工程研究》
CAS
北大核心
2018年第4期582-586,共5页
Chinese Journal of Tissue Engineering Research
