摘要
为深入了解小麦骨干亲本矮抗58及其衍生品种的遗传特性,利用84对SSR引物分析了47份品种(矮抗58及其衍生品种)的遗传多样性。结果表明,共检测到319个等位变异,每个SSR引物的等位变异数目为2~16个,平均为3.80个;多态性信息含量(PIC)变异范围为0.200~0.966,平均为0.559。3个基因组SSR位点平均等位变异丰富度分别为3.39、4.54和3.46(B>D>A),平均遗传多样性指数为0.543、0.627和0.508(B>A>D),B基因组的遗传多样性最丰富。7个部分同源群中,第7群SSR位点平均等位变异丰富度和遗传多样性指数均表现最高,分别为4.50和0.590。47份品种间的遗传相似性系数(G_(s))在0.544~0.922之间,平均为0.734,30个品种G;高于平均值,表明品种间遗传差异较小。非加权平均法(UPGMA)聚类结果表明,在G_(s)0.740处,47份品种被分为六类,其中38份(80.9%)品种被聚在Ⅰ类。
To understand the genetic characteristics of Aikang 58 and its derivatives, the genetic diversity of 47 Aikang 58 and its derivatives were analyzed using 84 pairs of SSR primers. A total of 319 alleles were detected, and the number of alleles per SSR primer ranged from 2 to 16, with an average of 3.80. The variation of polymorphic information content(PIC) ranged from 0.200 to 0.966, with an average of 0.559. The average allelic richness of the 3 SSR loci was 3.39, 4.54 and 3.46(B>D>A), and the average genetic diversity index was 0.543, 0.627 and 0.508(B>A>D). The genetic diversity of B genome was the most abundant. The average allelic diversity and genetic diversity index of SSR locus in group 7 were 4.50 and 0.590, respectively. The genetic similarity coefficient(G_(s)) of 47 cultivars of Aikang 58 and its derivatives ranged from 0.544 to 0.922, with an average of 0.734. The average value of(G_(s)) of 30 cultivars was higher than the average value, indicating that the genetic difference between cultivars was small. The results of unweighted average(UPGMA) clustering showed that 47 cultivars with Aikang 58 and their derivatives were divided into six categories at(G_(s)) 0.740, of which 38(80.9%) were clustered in class I.
作者
王晨晨
王玉泉
张海惠
卜明娜
张然
张金龙
胡喜贵
WANG Chenchen;WANG Yuquan;ZHANG Haihui;BU Mingna;ZHANG Ran;ZHANG Jinlong;HU Xigui(School of Life Science and Technology,He’nan Institute of Science and Technology,Xinxiang He’nan 453003,China;Center of Wheat Research,He’nan Institute of Science and Technology,Xinxiang He’nan 453003,China;Xinke College of He’nan Institute of Science and Technology,Xinxiang He’nan 453003,China)
出处
《种子》
北大核心
2021年第11期137-143,F0003,共8页
Seed
基金
河南省科技攻关项目(202102110161)
河南省高校重点科研项目(21 A 210007)
河南科技学院大学生创新训练项目(2019 CX 008)。
