摘要
【目的】探明茶树品种"紫娟"及诱变后代的遗传多样性。【方法】对选定的"紫娟"及"紫娟"诱变后代材料开展植物学性状调查、生化成分分析及EST-SSR标记研究。【结果】通过植物性状调查,有15份材料芽叶色泽与"紫娟"相近,成熟叶片的叶形只有2份材料与"紫娟"相同,为披针形,其余的为椭圆形或长椭圆形;叶色与"紫娟"相同的有9份材料,都为紫绿色,有7份材料叶色为黄绿色;春、夏两季生化成分的变异系数最高的为花青素,春季达到72.16%,夏季达到77.86%,花青素含量变化夏季大于春季;通过微卫星标记,共检测出165个等位基因,平均等位基因数为7.5个,有效等位基因数为2.562,Shannon信息指数为0.997,多态性信息含量为0.492,观测杂合度为0.629,期望杂合度为0.565。【结论】无论从性状、生理生化还是微卫星标记分析,"紫娟"的诱变后代都具有较为丰富的遗传多样性。
【Objective】The purpose of this paper was to explore the genetic diversity of the tea variety’Zijuan’and its mutagenesis progeny.【Method】The selected’Zijuan’and’Zijuan’mutated progeny were investigated from several aspects of botanical characters,biochemical components and EST-SSR markers.【Result】It was found that the color of buds and leaves of 15 materials were similar to that of’Zijuan’,only two materials in the leaf shape of mature leaves that were the same as’Zijuan’,which was lanceolate,and the others were elliptic or long elliptic.There were 9 materials with the same leaf color as’Zijuan’,all of which are purple green,while 7 materials were yellow-green.Among the variation coefficients of biochemical components in spring and summer,the highest variation coefficient was anthocyanin,which reached 72.16%in spring and 77.86%in summer.The content of anthocyanin changed more in summer than in spring.A total of 165 alleles were detected by microsatellite markers,the average number of alleles was 7.5,the effective number of alleles was 2.562,Shannon information index was 0.997,polymorphic information content was 0.492,observed heterozygosity was 0.629,and expected heterozygosity was 0.565.【Conclusion】The above analysis results of traits,physiological and biochemical characteristics and microsatellite markers showed that the mutant progeny of’Zijuan’had a relatively rich genetic diversity.
作者
陈春林
田易萍
邓少春
徐丕忠
仝佳音
尚卫琼
CHEN Chun-lin;TIAN Yi-ping;DENG Shao-chun;XU Pi-zhong;TONG Jia-yin;SHANG Wei-qiong(Tea Research Institute,Yunnan Academy of Agricultural Sciences,Yunnan Menghai 666201,China;Yunnan Technology Engineering Research Center of Tea Getnplasm Innovation and Suppoting Cultivation,Yunnan Menghai 666201,China;Yunnan Key Laboratoc of Tea Science,Yunnan Menghai 666201,China)
出处
《西南农业学报》
CSCD
北大核心
2021年第10期2108-2116,共9页
Southwest China Journal of Agricultural Sciences
基金
云南省科技厅项目“特异茶树‘紫娟’诱变后代的多样性研究”(2018FD130)。
关键词
“紫娟”
诱变
后代
遗传多样性
’Zijuan’
Mutation
Future generations
Genetic diversity
