摘要
通过水相滴定法制备蛇油纳米乳,研究了混合油相、表面活性剂、助表面活性剂对蛇油纳米乳制备的影响,并利用伪三元相图法确定最佳蛇油纳米乳配方。结果表明:混合油相选择蛇油和肉豆蔻酸异丙酯(IPM),表面活性剂选择Span80和Tween80二者质量比27∶73,助表面活性剂选择PEG400;最佳蛇油纳米乳配方为蛇油1.6 g、IPM 2.4 g、Span801.08 g、Tween802.92 g、PEG4004.0 g、水8.0 g。所制得的蛇油纳米乳为O/W型,平均粒径为63.01 nm,PDI为0.226,Zeta电位为-19 mV,液滴外观为圆形,粒径均匀且状态良好;离心后清澈透明,不分层;不耐高温,需要在25℃以下贮存;冷冻后,可以在室温下恢复到初始状态;氧化稳定性较蛇油提高。
The snake oil nanoemulsion was prepared by water phase titration.The effects of mixed oil phase,surfactant and cosurfactant on the preparation of snake oil nanoemulsion were studied.The pseudo three phase diagram method was used to determine the optimal formula of snake oil nanoemulsion.The results showed that snake oil and isopropyl myristate(IPM)were selected as the mixed oil phase,Span80 and Tween80 with mass ratio of 27∶73 as the surfactants,PEG400 as the cosurfactant.The optimal formula of smake oil nanoemulsion was obtained as follows:snake oil 1.6 g,IPM 2.4 g,Span801.08 g,Tween802.92 g,PEG4004.0 g and water 8.0 g.The snake oil nanoemulsion was O/W type.The average partical size,PDI and Zeta potential of snake oil nanoemulsion were 63.01 nm,0.226 and-19 mV,respectively,and the droplet appearance was round,the partical size was uniform and in good state.After centrifugation,snake oil nanoemulsion was clear and transparent without stratification.It was not resistant to high temperature and needed storing below 25℃.After freezing,it could be restored to its original state at room temperature.Its oxidation stabilty was higher than snake oil.
作者
李藤藤
徐东升
吴迪
李琪
娄小倩
李相军
任立群
LI Tengteng;XU Dongsheng;WU Di;LI Qi;LOU Xiaoqian;LI Xiangjun;REN Liqun(Department of Experimental Pharmacology and Toxicology,School of Pharmacy,Jilin University,Changchun 130021,China;Department of Oncology,The First Hospital of Jilin University,Changchun 130000,China;Department of Pathology,The Third Affiliated Hospital of Jinzhou Medical University,Jinzhou 120001,Liaoning,China;Department of Endocrinology,The First Hospital of Jilin University,Changchun 130021,China)
出处
《中国油脂》
CAS
CSCD
北大核心
2021年第11期21-25,49,共6页
China Oils and Fats
基金
吉林省重点科技研发项目(20180201007YY)。
关键词
蛇油
纳米乳
制备
稳定性
粒径
PDI
ZETA电位
snake oil
nanoemulsion
preparation
stability
particle size
PDI
Zeta potential
