摘要
通过正交试验优化了党参中醇溶性多糖的提取工艺,并对其生物活性进行了研究。最佳提取条件固液比为1∶20,提取温度为65℃,提取时间为4.5 h,为超声波辅助提取,提取次数为2次,在这些条件下,党参多糖的产率为26.25±1.02%。分析表明,党参多糖中,总糖、蛋白质、糖醛酸含量分别为805、31~66、2.1 g/kg。体外抗氧化实验结果表明,在浓度为2 mg/mL时,党参多糖对ABTS+、DPPH、和HO-具有良好的清除作用。对不同区域党参多糖进行含量比较,认为降水量充足、冷凉的气候、较高的海拔有利于糖分积累。
The extraction process of alcohol-soluble polysaccharide on Codonopsis pilosula was optimized by orthogonal tests,and its biological activity was studied.The optimal extraction conditions were the ratio of solid-to-liquid was 1∶20 g/mL,temperature 65℃,extraction time 4.5 h,and ultrasonic assisted extraction cofactor and extraction number was two times,polysaccharide yield reached 26.25±1.02%.Chemical composition analysis showed that the total sugar,protein and glyuronate content of Codonopsis pilosula polysaccharide were 805 g/kg,31~66 g/kg and 2.1 g/kg,respectively.In vitro antioxidant assays demonstrated that Codonopsis pilosula polysaccharide had good clearance on ABTS+,DPPH and HO-at a concentration of 2 mg/mL.By comparing the content of codonopsis pilotica polysaccharide in different regions,sufficient rainfall,cold climate and high altitude were conducive to polysaccharide accumulation.
作者
徐美蓉
李晓蓉
丁文姣
陶海霞
漆永红
李婷
XU Meirong;LI Xiaorong;DING Wenjiao;TAO Haixia;QI Yonghong;LI Ting(Institute of Agricultural Quality Standards and Testing Technology,Gansu Academy of Agricultural Sciences,Lanzhou Gansu 730070,China;Animal Husbandary,Pasture and Green Agriculture Institute,Gansu Academy of Agricultrual Sciences,Lanzhou Gansu 730070,China;Institute of Plant Protection,Gansu Academy of Agricultural Sciences,Lanzhou Gansu 730070,China)
出处
《甘肃农业科技》
2021年第11期4-11,共8页
Gansu Agricultural Science and Technology
基金
国家重点研发项目(2018YFD0201100)
甘肃省重点人才项目(2019RCXM072)
甘肃省农业科学院重点研发计划(2020GAAS30、2020 GAAS25)。
关键词
党参多糖
提取工艺
优化
生物活性
Codonopsis pilosula polysaccharide
Extraction process
Optimization
Biological activity
