摘要
本文采用分光光度法和琼脂扩散法,初步研究了一株海洋源长枝木霉菌ST-27的发酵产物对无乳链菌的抑制作用,结果显示该发酵产物的二氯甲烷萃取物对无乳链球菌的抑制作用明显,最小抑菌浓度(MIC)值为0.312 mg/mL;实验表明,该萃取物对无乳链球菌的生物被膜形成具有重要影响,进一步通过扫描电镜发现二氯甲烷萃取物作用后的无乳链球菌,其大部分细胞表面有明显褶皱甚至出现破裂;与临床上常见的抗生素的抑菌效果比较,二氯甲烷萃取物的抑菌活性有明显的优势。
In this paper,the inhibition of the fermentation product of Trichoderma longibrachiatum ST-27 on Streptococcus agalactiae was studied by a spectrophotometry and agar diffusion method.The results showed that the dichloromethane extract from T.longibrachiatum ST-27 fermentation product had obvious inhibitory effect on S.agalactiae,and the minimum inhibitory concentration(MIC)value was 0.312 mg/mL.It was found that the extract had an important effect on the biofilm formation of S.agalactiae.From scanning electron microscope(SEM)images,it was observed that most of the cells of S.agalactiae treated with dichloromethane extract had obvious folds or even ruptures on their surface.Compared with the clinical common antibiotics,the extraction of dichloromethane has obvious advantages in antimicrobial activity.As a substitute for traditional antibiotics,the results of this study have great application potential in inhibiting S.agalactiae.
作者
苏艳君
郑诗婷
吴鹏
唐旭
SU Yan-jun;ZHENG Shi-ting;WU Peng;TANG Xu(Stomatological Hospital of Xiamen Medical College,Xiamen 361006,China;Technology Innovation Center for Exploitation of Marine Biological Resources,Third Institute of Oceanography,Ministry of Natural Resources,Xiamen 361005,China)
出处
《天然产物研究与开发》
CAS
CSCD
2021年第11期1918-1924,共7页
Natural Product Research and Development
基金
厦门市海洋经济创新发展示范项目(16CZY009SF05)
厦门南方海洋研究中心项目(21CZP003HJ13)。
关键词
长枝木霉菌
发酵产物
无乳链球菌
抑菌活性
Trichoderma longibrachiatum
fermentation product
Streptococcus agalactiae
antibacterial activity
