摘要
何首乌有生首乌和制首乌之分,生首乌能解毒,润肠通便;制首乌能补肝肾,益精血,乌须发,强筋骨,化浊降脂。生/制首乌都含有二苯乙烯苷类、蒽醌类、磷脂类等化学成分,但其含量存在一定差异。生首乌炮制后其毒性减弱。生/制首乌的功效、主治和毒性都存在一定的差异,在外观性状上差异较易识别,但其磨成粉末后两者不易区分。因此,寻找一种快速简单的判别方法来区分生/制首乌十分必要。中红外光谱法具有检测速度快,对样品无损等优势,已在中药质量控制和鉴别中得到广泛的应用。该研究建立生/制首乌中红外光谱的指纹图谱,并采用正交偏最小二乘-判别分析对其进行鉴别。在4000~700 cm^(-1)范围内采集38批不同来源生/制首乌中红外光谱数据,分析其主要的特征峰,并用simca13.0软件对数据进行正交偏最小二乘-判别分析。建立38批不同来源生/制首乌中红外指纹图谱,并分析出主要化学成分包括蛋白质类、核酸类、脂肪酸类、蒽醌类、二苯乙烯苷类、磷脂类。对红外光谱峰形和峰强度进行分析,生首乌和制首乌的峰形差异比较少,但峰强度具有一定的差异。为了建立生/制首乌红外光谱差异模型,采用正交偏最小二乘-判别分析法,其结果能很好地将生/制首乌分成两类,左边为制首乌,右边为生首乌。根据变量在项目中的重要性(VIP值)筛选差异化学成分,并利用SPSS17.0统计软件进行t检验,以VIP>1及p<0.05确定差异性化学成分。生/制首乌的差异性化学成分为二苯乙烯苷类、蒽醌类、磷脂类,表明何首乌炮制后二苯乙烯苷类、蒽醌类、磷脂类的含量都发生变化。中红外光谱结合模式识别筛选出差异性化学成分和文献报道基本一致,表明此法可用于生/制首乌鉴别。本研究成功采用中红外光谱对生/制首乌进行快速检测和整体质量评价,结合模式识别能将其鉴别,可为中�
The roots of Polygonum multiflorum are traditional Chinese medicinal herbs in processed form or raw state.Raw Polygonum multiflorum can detoxify and loosen the bowel and relieves constipation,but processed Polygonum multiflorum can benefit blood,hair,strong muscles and bones,turbidness and lipid lowering.The raw and processed Polygonum multiflorum contain chemical components such as stilbene glycosides,anthraquinone and phospholipid,but their contents are different.The toxicity of raw Polygonum multiflorum decreased after processing.The chemical composition,efficacy and hepatotoxicity of raw and processed Polygonum multiflorum are different.It was easy to recognize the difference in appearance between raw and processed Polygonum multiflorum,but it was not easy to distinguish the power of raw and processed Polygonum multiflorum.Therefore,it is necessary to find a fast and simple method for distinguishing them.Mid-IR has the advantages of fast detection speed and nondestructive.Mid-IR has been widely used in the identification of traditional Chinese medicine.This paper aimsf to establish the fingerprint of the mid-infrared spectrum of raw and processed Polygonum multiflorum and identify them by orthogonal partial least-squares discriminant analysis(OPLS-DA).The chemical composition in 38 batches of raw and processed Polygonum multiflorum were determined by mid-IR of 4000~700 cm^(-1),and the characteristic peaks of chemical composition were analyzed.OPLS-DA of simca13.0 software analyzed the data.The mid-infrared fingerprints of 38 batches of polygonum multiflorum from different sources were established,mainly including protein,nucleic acid,fatty acid,anthraquinone,stilbene glycosides and phospholipids.The peak shape and peak intensity of the infrared spectrum were analyzed,and the difference of peak shape between the raw and the processed radix aconitum was less,but the peak strength was different.OPLS-DA was used to establish the infrared spectral difference model of raw/processed Polygonum multiflorum.The results
作者
林艳
夏伯候
李春
林丽美
李亚梅
LIN Yan;XIA Bo-hou;LI Chun;LIN Li-mei;LI Ya-mei(Key Laboratory for Quality Evaluation of Bulk Herbs of Hunan Province,Changsha 410208,China;Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China)
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2021年第12期3708-3711,共4页
Spectroscopy and Spectral Analysis
基金
湖南省自然科学基金项目(2020JJ6008)
湖南中医药大学校级科研基金项目(2020XJJJ019)
湖南省科技计划项目(2019XF5076)
湖南省教育厅项目(20C1433)
湖南中医药大学中药学一流学科项目(校行科字【2018】3号)资助。
关键词
中红外光谱
生首乌
制首乌
模式识别
二苯乙烯苷
Mid-infrared spectroscopy
Crude Polygonum multiflorum
Processed Polygonum multiflorum
Pattern recognition
Stilbene glycosides
