摘要
目的采用超高效液相色谱-四级杆飞行时间串联质谱(UPLC-Q-TOF-MS)技术分析百事乐胶囊中的化学成分。方法采用Waters ACQUITY UPLC BEH C_(18)(2.1 mm×100 mm,1.7μm)色谱柱,以乙腈-0.1%甲酸水溶液为流动相进行梯度洗脱,流速为0.4 mL·min^(-1),柱温为35℃,进样量为3μL;采用电喷雾离子源,在正、负离子模式下采集数据。运用MassLynx V4.1软件,根据化合物的相对保留时间、准分子离子峰和碎片离子峰等信息,结合对照品和相关文献资料进行化学成分分析。结果在百事乐胶囊中鉴定出了42个化学成分,主要包括有机酸类、黄酮类、皂苷类、萜类和姜黄素类等,并对化合物的药材来源进行了归属。结论建立的方法能够快速、准确、系统地分析百事乐胶囊多种化学成分,较全面地阐述了百事乐胶囊的化学成分信息,为该方的质量控制及药效物质基础研究提供了理论基础。
Objective To study the chemical constituents in Baishile Capsules by ultra-performance liquid chromatography-quadrupole-time of flight-mass spectrometry(UPLC-Q-TOF-MS).Methods The separation was performed on an Waters ACQUITY UPLC BEH C_(18)(2.1 mm×100 mm,1.7μm)by a gradient elution of acetonitrile-0.1%formic acid solution.The flow rate was 0.4 mL·min^(-1)and the column temperature was 35℃,the injection volume was 3μL.Electrospray ionization was applied and the data were collected via positive and negative ion modes.By using MassLynx V4.1 software,the chemical constituents were analyzed based on the retention time,excimer ion peak and fragment ion peak of the compounds,as well as comparison with reference substances and literature data.Results A total of 42 chemical constituents in Baishile Capsules were identified,including organic acids,flavonoids,saponins,terpenoids and curcumin,and the medicinal sources of the compounds were assigned.Conclusion The established method can quickly,accurately and systematically identify various chemical components of Baishile Capsules.The information of chemical constituents in Baishile Capsules is comprehensively expounded.The study establishes a foundation for the research of quality control and material foundation of efficacy of Baishile Capsules.
作者
雷昌
凌成利
陈威
杨振宇
张益铭
陈琦松
王宇红
黄丹
LEI Chang;LING Cheng-li;CHEN Wei;YANG Zhen-yu;ZHANG Yi-ming;CHEN Qi-song;WANG Yu-hong;HUANG Dan(Hunan University of Chinese Medicine/Technology Innovation Center,Hunan University of Chinese Medicine,Changsha 410208,China;Hunan Key Laboratory of Powder and Innovative Drugs State Key Laboratory of Ministry Training Bases,Changsha 410208,China)
出处
《时珍国医国药》
CAS
CSCD
北大核心
2021年第8期1904-1908,共5页
Lishizhen Medicine and Materia Medica Research
基金
国家自然科学基金青年基金(81603608)
湖南省自然科学基金青年基金(2020JJ5417)
中医内科重大疾病防治研究与转化教育部重点实验室开放基金(ZYNK201708)
湖南中医药大学中医学一流学科开放基金(2018ZYX48)
湖南中医药大学大学生创新创业训练计划项目(X201910541045,X202010541129)
湖南中医药大学中医学国内一流建设学科(2018年)。
