摘要
细胞色素P450酶(cytochrome P450)广泛参与植物次生代谢,是当前的研究热点。基于苦蘵转录组数据,分析并鉴定了部分苦蘵P450家族基因。共鉴定得到795个可能为P450家族基因的序列片段,并将它们聚类到25个不同的聚类(cluster)中,其中,有12个聚类具有组织特异性表达。最终拼接得到30个苦蘵P450家族基因的全长序列,它们与其他物种P450基因高度同源。进化分析表明,这30个苦蘵P450基因可归为9个P450亚家族。通过定量PCR技术,分析了多个P450基因在果实不同成熟阶段的表达变化。结果表明,9个P450基因随着果实成熟表达量上升,另外9个P450基因表达量则逐渐降低。以果实为材料,分析了P450家族基因在乙烯(ACC)与乙烯抑制剂(1-甲基环丙烯,1-MCP)处理下的表达变化。研究表明,部分P450家族基因在ACC和1-MCP处理下表现出相反的表达模式,这说明P450可能参与乙烯介导的果实成熟过程。克隆了3个果实特异表达的P450家族基因(comp164210、comp131532、comp152181),它们主要定位于细胞质和细胞膜中。本研究为苦蘵药用性状遗传改良提供了有价值的遗传信息。
Cytochrome P450 enzymes are widely involved in plant secondary metabolism.Based on the transcriptome data of Physalis angulata,several P450 family members had been identified.795 possible sequence fragments of P450 gene had been identified,and they were clustered into 25 different clusters,including 12 clustering with tissue-specific expression.By bioinformatics splicing,full-length sequence of 30 Physalis angulata P450 gene family,which was highly homologous with other P450 species genes,were obtained.Phylogenetic analysis showed that 30 P450 genes could be classified into nine P450 subfamilies.By qRT-PCR method,expression of 30 P450 genes under different developmental stages of fruits was analyzed.The expression of nine P450 genes increased with fruit maturation,while expression of other nine P450 genes decreased gradually.The expression of P450 family genes under ethylene(ACC)and ethylene inhibitor(1-methylcyclopropene,1-MCP)treatments were also analyzed.Part of the P450 family genes showed the opposite expression pattern under ACC and 1-MCP treatments,suggesting P450 family genes might be involved in ethylene-mediated fruit ripening process.Further,three fruit-specific expressed P450 family genes(comp164210,comp131532,comp152181)had been cloned,and they were mainly located in the cytoplasm and cell membrane.This study provided valuable genetic information for the genetic improvement of medicinal characters of P.angulata.
作者
蒋智芳
韩怡蝶
楼盼盼
郭宏
冯尚国
沈晨佳
王慧中
JIANG Zhifang;HAN Yidie;LOU Panpan;GUO Hong;FENG Shangguo;SHEN Chenjia;WANG Huizhong(College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China;Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants, Hangzhou 311121, China)
出处
《浙江农业学报》
CSCD
北大核心
2021年第11期2009-2016,共8页
Acta Agriculturae Zhejiangensis
基金
国家自然科学基金(31970346)
浙江省自然科学基金(LY20H280012,LY19C160001)。
关键词
细胞色素P450酶
苦蘵
转录组
乙烯
cytochrome P450 enzyme
Physalis angulate
transcriptome
ethylene
