摘要
目的研究循环肿瘤细胞(circulating tumor cells,CTC)在血液中的运动过程以及Cell Collector取样针对CTC的捕获机理,揭示CTC检出数量与体内实际浓度的关系。方法基于Fluent和EDEM软件,应用单向流固耦合的两相流计算方法,建立血流动力学模型及CTC组分运输模型,开展不同CTC浓度工况下的捕获特性仿真。结果 Cell Collector取样针的CTC捕获数量与体内CTC浓度呈明显正相关。CTC浓度较低时,只能在少数时间区间内捕获到CTC颗粒,捕获有一定的偶然性;随着浓度增加,CTC捕获数在时间上的均匀性变好,同时捕获总数也随之增加。结论通过拟合仿真结果,初步获得CTC捕获数量与体内浓度的量化解析关系,为Cell Collector的临床使用提供理论依据及力学机制阐释。
Objective To study movement process of circulating tumor cells( CTCs) in the blood and mechanism of CTC capture by Cell Collector,and reveal relationship between the detected CTC numbers and the actual CTC concentration in the body. Methods Based on Fluent and EDEM software,the unidirectional fluid-solid interaction method was applied to establish a two-phase flow model,including the hemodynamic model and the CTC transport model,and capture simulations under different CTC concentration conditions were conducted. Results The number of CTCs captured by Cell Collector was significantly positively correlated with the CTC concentration in the body. When the CTC concentration was low,CTCs could only be captured in several time intervals,and the capture had a certain contingency;as the concentration increased,the uniformity of CTC capture over time became better,and the total number of captures also increased. Conclusions Through the fitting of simulation results,analytical quantitative relationship between the captured CTC number and the CTC concentration in the body is preliminarily given,which provides theoretical basis and mechanical explanation for the clinical use of Cell Collector.
作者
王检耀
庄淳
齐世霏
王鸿东
WANG Jianyao;ZHUANG Chun;QI Shifei;WANG Hongdong(State Key Laboratory of Ocean Engineering,Shanghai Jiao Tong University,Shanghai 200240,China;Key Laboratory of Marine Intelligent Equipment&System of Ministry of Education,Shanghai Jiao Tong University,Shanghai 200240,China;School of Biomedical Engineering,Shanghai Jiao Tong University,Shanghai 200240,China;Department of Gastrointestinal Surgery,Renji Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200120,China)
出处
《医用生物力学》
CAS
CSCD
北大核心
2021年第5期783-789,共7页
Journal of Medical Biomechanics
基金
上海交通大学医工交叉研究基金(ZH2018QNA45)。
关键词
循环肿瘤细胞
单向流固耦合
两相流
数值仿真
circulating tumor cell(CTC)
unidirectional fluid-structure interaction
two-phase flow
numerical simulation
