摘要
目的探究毛尖、铁观音、普洱3种茶叶水提物的体外抗氧化作用及对H_(2)O_(2)诱导的RAW264.7细胞损伤的保护作用。方法用福林酚法测定3种提取物中茶多酚含量。采用1,1-二苯基-2-三硝基苯肼(DPPH)法和铁离子还原(FRAP)法对3种茶多酚提物的体外抗氧化活性进行研究。采用噻唑兰比色法(MTT)研究3种茶多酚提物对H_(2)O_(2)诱导的RAW264.7巨噬细胞损伤的保护作用。结果毛尖、铁观音、普洱茶多酚提取物的茶多酚含量分别为(46.79±0.99)%、(46.22±1.03)%、(33.72±0.60)%。毛尖、铁观音、普洱各自水提物的DPPH自由基半数清除浓度(IC_(50))分别为4.33、4.91、13.49mg/L。毛尖、铁观音、普洱水提物的FRAP值分别为(350.89±3.11)、(261.55±2.98)、(109.97±3.25)mmol/L。3种茶叶的水提物在浓度低于80mg/L时,均对H_(2)O_(2)诱导RAW264.7细胞氧化应激有良好的保护作用。结论茶多酚提取物的DPPH自由基清除能力及FRAP值的大小关系均为:毛尖>铁观音>普洱。提取物的抗氧化活性也与茶多酚含量呈正相关。3种茶叶的水提物在80mg/L浓度以内均对H_(2)O_(2)诱导的RAW264.7细胞氧化应激有保护作用。
Objective To explore the in vitro antioxidant effects of water extracts of Maojian tea,Tieguanyin tea,and Puer tea and their protective effects on hydrogen peroxide(H_(2)O_(2))induced injury in RAW264.7 cells.Methods The contents of tea polyphenols in the three extracts were determined by Folin colorimetry.The 1,1-diphenyl-2-trinitrophenylhydrazine(DPPH)method and the ferric reducing antioxidant power(FRAP)assay were used to evaluate the in vitro antioxidant activities of three tea polyphenols extracts.The protective effects of the three tea polyphenols extracts on H_(2)O_(2)induced injury in macrophages RAW264.7 cells were detected by MTT assay.Results The contents of tea polyphenols were(46.79±0.99)%,(46.22±1.03)%and(33.72±0.60)%in the polyphenols extracts of Maojian tea,Tieguanyin tea and Puer tea,respectively.The DPPH free radical scavenging concentrations(IC 50)were 4.33,4.91 and 13.49 mg/L in the water extracts of Maojian tea,Tieguanyin tea,and Puer tea,respectively.The FRAP values were(350.89±3.11),(261.55±2.98)and(109.97±3.25)mmol/L for the water extracts of Maojian tea,Tieguanyin tea and Puer tea,respectively.The water extracts of three kinds of tea exhibited good protective effects against H_(2)O_(2)induced oxidative stress in RAW264.7 cells when the concentrations were lower than 80 mg/L.Conclusions The DPPH free radical scavenging ability and FRAP value of tea polyphenols extract are Maojian tea>Tieguanyin tea>Puer tea.The antioxidant activity of the extract is also positively correlated with the tea polyphenol content.The water extracts of three kinds of tea have protective effects against H_(2)O_(2)induced oxidative stress in RAW264.7 cells.
作者
陈晨
何孝银
李凡
林奇泗
CHEN Chen;HE Xiaoyin;LI Fan;LIN Qisi(School of Pharmacy,Xuzhou Medical University,Xuzhou,Jiangsu 221004,China;Jiangsu Provincial Xuzhou Pharmaceutical Vocational College,Xuzhou,Jiangsu 221116)
出处
《徐州医科大学学报》
CAS
2021年第10期747-752,共6页
Journal of Xuzhou Medical University
基金
徐州市科技计划项目(KC19043)。
关键词
茶多酚
抗氧化活性
细胞损伤
二苯基-2-三硝基苯肼法
铁离子还原法
tea polyphenols
antioxidant activity
cell damage
1,1-diphenyl-2-trinitrophenylhydrazine
ferric reducing antioxidant power
