摘要
目的研究阿魏酸钠对神经病理痛大鼠痛觉的改善作用及对p38MAPK信号通路的影响。方法建立SD大鼠CCI模型,将40只SPF级健康SD雄性大鼠分为假手术组、模型组、阿魏酸钠组、p38MAPK抑制组,每组10只。观察各组大鼠疼痛行为学、脊髓背角5-HT、GABA浓度及p38MAPK、p-p38MAPK、CREB、p-CREB蛋白变化。结果阿魏酸钠组及p38MAPK抑制组建模后MWT及脊髓背角5-HT、GABA浓度均低于假手术组而高于模型组,TWL短于假手术组而长于模型组,p-p38MAPK/p38MAPK、p-CREB/CREB均高于假手术组而低于模型组,差异均有统计学意义(P<0.05),阿魏酸钠组及p38MAPK抑制组上述指标比较,差异无统计学意义(P>0.05)。结论阿魏酸钠能抑制大鼠神经病理痛,其机制可能与抑制p38MAPK信号通路的激活有关。
Objective To study the effect of sodium ferulate on improving pain perception in rats with neuropathic pain and its influence on p38MAPK signal pathway.Methods SD rats established CCI model,which were divided into sham operation group,model group,sodium ferulate group,and p38MAPK inhibition group.Observe the pain behavior,5-HT,GABA concentration of spinal dorsal horn and the changes of p38MAPK,p-p38MAPK,CREB,and p-CREB protein in each group.Results The MWT and 5-HT,GABA concentrations in spinal dorsal horn in sodium ferulate group and p38MAPK inhibition group were lower than those in sham operation group but higher than those in model group.TWL was shorter than those in sham operation group but longer than those in model group and longer than those in model group,P-P38MAPK/p38MAPK and p-CREB/CREB were higher in sodium ferulate group and lower than those in model group(P<0.05).There was no significant difference in the above indicators between sodium ferulate group and p38MAPK inhibition group(P>0.05).Conclusion Sodium ferulate can inhibit neuropathic pain in rats,and the mechanism may be related to the inhibition of p38MAPK signal pathway activation.
作者
张爱霞
卢晶
吴饶平
ZHANG Aixia;LU Jing;WU Raoping(Department of Basic Discipline, Jiangxi Health Vocational College, Nanchang 330052, China)
出处
《西部医学》
2021年第11期1591-1595,共5页
Medical Journal of West China
基金
江西省教育厅青年科学基金(GJJ12679)。
