摘要
富含亮氨酸重复序列型类受体蛋白激酶(LRR-RLK)在植物生长发育、病原防御以及环境适应等多个方面发挥重要作用。为了研究菘蓝(Isatis tinctoria)中LRR-RLK的表达特征,采用cDNA末端快速扩增(RACE)技术克隆菘蓝LRR-RLK编码基因IiLTK的全长cDNA,并获得其基因组DNA序列。IiLTK基因全长cDNA为2477 bp(GenBank登录号DQ211577),包含1个2160 bp的开放阅读框(ORF),编码719个氨基酸残基。对应的基因组分析表明,IiLTK基因含15个内含子和16个外显子。通过qRT-PCR检测IiLTK在四倍体和二倍体菘蓝的不同器官中及受到不同胁迫条件下的表达情况:IiLTK基因在2种倍性菘蓝的根、茎和叶中均有表达,且四倍体菘蓝根和叶中的表达水平均显著高于二倍体。赤霉素(GA3)和脱落酸(ABA)处理能显著提高IiLTK的表达水平;高盐(NaCl)和茉莉酸甲酯(MeJA)处理显著降低IiLTK的表达水平;低温(4℃)和水杨酸(SA)处理对IiLTK表达水平影响不大。
Leucine-rich repeat receptor-like kinases(LRR-RLK) play an important role in plant growth and development, pathogen defense and environmental adaptation. In order to investigate the expression characteristics of LRR-RLK in Isatis tinctoria, the full-length cDNA of I. tinctoria LRR-RLK gene Ii LTK was cloned by rapid amplification of cDNA end(RACE), and its genomic DNA sequence was obtained. The full-length cDNA of IiLTK gene is 2 477 bp(GenBank accession number DQ211577) long with an open reading frame(ORF) of 2 160 bp encoding a polypeptide of 719 amino acid residues. The corresponding genome analysis showed that the IiLTK gene contained 15 introns and 16 exons. The expression of IiLTK in different organs of tetraploid and diploid I. tinctoria and under different stress conditions was detected by qRT-PCR: IiLTK gene was expressed in the roots, stems and leaves of both tetraploid and tetraploid I. tinctoria, and its expression level in roots and leaves of the tetraploids I. tinctoria was significantly higher than that of its diploid counterpart. Gibberellin(GA3) and abscisic acid(ABA) treatments could significantly upregulate the expression level of Ii LTK, salt(NaCl) and methyl jasmonate(MeJA) could significantly downregulate its expression, whereas cold(4℃) and salicylic acid(SA) could not regulate its expression level obviously.
作者
李姝诺
杨颖博
马雪祺
李元玉
张国宁
陈万生
肖莹
LI Shunuo;YANG Yingbo;MA Xueqi;LI Yuanyu;ZHANG Guoning;CHEN Wansheng;XIAO Ying(Research and Development Center of Chinese Medicine Resources and Biotechnology,Institute of Traditional Chinese Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Medical Guarantee Center,Changzheng Hospital,Naval Medical University,Shanghai 200003,China;Department of TCM Processing,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Shanghai Tufeng Pharmaceutical Technology Company Limited,Shanghai 201203,China)
出处
《植物生理学报》
CAS
CSCD
北大核心
2021年第9期1715-1726,共12页
Plant Physiology Journal
基金
国家自然科学基金(81874335和31872665)
上海市青年科技启明星计划(18QB1402700)
上海市中央引导地方科技发展资金项目(YDZX20203100002948)。
关键词
菘蓝
类受体蛋白激酶
基因克隆
生物信息学
表达分析
Isatis indigotica
receptor-like protein kinase
gene cloning
bioinformatics
expression analysis
