摘要
背景:间充质干细胞替代疗法一直被认为是治疗纤维化疾病的有效方法之一,但安全性受到质疑,而外泌体是间充质干细胞的旁分泌产物,与间充质干细胞的疗效相似,且作为一种无细胞治疗方法,目前已成为研究者们关注的重点。目的:将间充质干细胞源性外泌体在肺、肝、心脏、肾纤维化修复中的作用进行总结,对外泌体用于修复放射性唾液腺纤维化进行展望。方法:在PubMed数据库中以“exosomes,mesenchymal stem cells,tissue fibrosis,radiation salivary glands,repair”为关键词进行相关文献检索,在CNKI数据库中以“外泌体,间充质干细胞,组织纤维化,放射性唾液腺,修复”为关键词进行检索,通过快速浏览文章题目及摘要进行筛选,排除与主题关系不密切的文章,最终筛选出46篇文献进行精读并撰写。结果与结论:外泌体主要由脂质、蛋白质、核酸等构成,具有细胞通讯、抗细胞凋亡、抗炎、抗纤维化等作用,其提取方法多样,各具优劣势。间充质干细胞源性外泌体在修复肺、肝、心脏、肾纤维化损伤疾病中起到重要作用,但运用于修复放射性唾液腺损伤引起的组织纤维化研究较少,值得深入研究和探索。
BACKGROUND:Mesenchymal stem cell replacement therapy has always been regarded as one of effective methods for the treatment of fibrotic diseases.Its safety has been questioned.Exosomes are the paracrine products of mesenchymal stem cells with similar curative effects.This kind of cell-free therapy has now become the focus of researchers.OBJECTIVE:To summarize the role of mesenchymal stem cell-derived exosomes in the repair of lung,liver,heart and kidney fibrosis,and to look into the application of exosomes in the repair of radioactive salivary gland fibrosis.METHODS:PubMed database and CNKI database were retrieved with“exosomes,mesenchymal stem cells,tissue fibrosis,radiation salivary glands,repair”as keywords.Articles were filtered by a quick look at the article title and abstract.The articles that were not closely related to the topic were excluded.This paper finally selected 46 articles in intensive reading and writing.RESULTS AND CONCLUSION:Exosomes are mainly composed of lipids,proteins,nucleic acids,which have the functions of cell communication,anti-apoptosis,anti-inflammatory,and anti-fibrosis.The extraction methods are diverse and each has its advantages and disadvantages.Mesenchymal stem cell-derived exosomes play an important role in repairing fibrosis damage of the lung,liver,heart,and kidney.However,there are few studies on repairing tissue fibrosis caused by radioactive salivary gland damage,and it is worthy of in-depth study and exploration.
作者
唐建宏
张霓霓
黄桂林
郎家婵
崔田宁
骆勤亮
Tang Jianhong;Zhang Nini;Huang Guilin;Lang Jiachan;Cui Tianning;Luo Qinliang(Zunyi Medical University,Zunyi 563000,Guizhou Province,China;Department of Oral and Maxillofacial Surgery,Affiliated Stomatological Hospital of Zunyi Medical University,Zunyi 563000,Guizhou Province,China)
出处
《中国组织工程研究》
CAS
北大核心
2022年第25期4022-4027,共6页
Chinese Journal of Tissue Engineering Research
基金
国家自然科学基金(81860198),项目负责人:张霓霓
国家自然科学基金(81960204),项目负责人:黄桂林
贵州省卫生健康委科学技术基金项目(gzwjkj2020-1-165),项目参与人:黄桂林、张霓霓、唐建宏。
关键词
干细胞
间充质干细胞
外泌体
纤维化
唾液腺
放射
修复
综述
stem cells
mesenchymal stem cells
exosomes
fibrosis
salivary glands
radiation
repair
review
