摘要
软骨组织工程是未来修复关节软骨的重要策略,但面临着软骨细胞数量不足,体外培养去分化以及分化方向难以控制等瓶颈。共培养体系的提出为这些问题提供了新的解决方式。一系列的研究发现,共培养体系可以阻止软骨细胞在体外培养的去分化现象,并促使已发生去分化的软骨细胞实现再分化,同时促进体系中干细胞的软骨分化以及抑制其肥大化进程。本综述通过对国内外应用在软骨组织工程的共培养研究进行概述,总结共培养体系对于软骨组织工程软骨形成的作用,同时探讨其积极作用的机制。
Cartilage tissue engineering appears as an important strategy for repairing articular cartilage. However,it faces the problems of insufficient number of chondrocytes,dedifferentiation in vitro,and difficulty in controlling the direction of differentiation. The co-culture system provides a new solution to these problems. A series of studies have reported that the co-culture system can prevent the dedifferentiation of chondrocytes cultured in vitro,promote the re-differentiation of dedifferentiated chondrocytes,and facilitate the chondrogenic differentiation of stem cells in the system as well as inhibit the process of hypertrophy. This review summarized the literatures about the application of co-culture system in cartilage tissue engineering,discussed the role of co-culture system in cartilage tissue engineering, and explored the underlying mechanism.
作者
邹健宇
刘日许
郑仕聪
姚咏嫦
Zou Jianyu;Liu Rixu;Zheng Shicong;Yao Yongchang(Department of Joint Surgery,The First Affiliated Hospital of Guangzhou Medical University,Guangzhou 510120,China;Guangdong key laboratory of orthopaedic technology and implant materials,The First Affiliated Hospital of Guangzhou Medical University,Guangzhou 510120,China)
出处
《中华关节外科杂志（电子版）》
CAS
2018年第6期835-841,共7页
Chinese Journal of Joint Surgery(Electronic Edition)
基金
广州市属高校科研项目重点项目(1201610097)
广州市科技计划项目一般项目(201804010479)
关键词
共同培养技术
软骨细胞
组织工程
Coculture techniques
Chondrocytes
Tissue engineering
