摘要
目的研究告达庭(caudation)对卵巢癌生长的抑制作用及其机制。方法细胞实验分为两组,即对照组和告达庭组(30μmol/L告达庭作用卵巢癌细胞SKOV324h)。CCK-8法检测细胞增殖;ELISA法检测细胞凋亡;Western blot法检测SKOV3细胞中DNMT1、Axin、Bcl-2、cyclinD1蛋白的表达。观察告达庭对卵巢癌细胞内凋亡相关蛋白的表达作用。建立起裸鼠卵巢癌皮下移植瘤模型,观察移植瘤的生长,实时荧光定量PCR检测凋亡相关基因的表达。结果与对照组比较,告达庭可抑制卵巢癌细胞SKOV3增殖,并促进癌细胞凋亡,呈浓度依赖性;告达庭干预后,DNMT1、Bcl-2及cyclinD1表达均下降,Axin表达上调。在体外移植瘤实验中,告达庭可抑制移植瘤的生长,并抑制卵巢癌组织内DNMT1、Bcl-2及cyclinD1基因表达,上调Axin基因表达。结论告达庭可抑制卵巢癌生长,其机制可能与调控DNMT1/β-catenin信号通路有关。
Objective To study the inhibitory effect of Caudatin on growth of ovarian cancer and its mechanism.Methods The cell experiment was divided into two groups.Caudatin group was treated with 30μmol/L Caudatin for 24 h,while control group normal saline.Cell viability was detected by the CCK-8 assay and apoptosis was measured by ELISA.DNMT1,Axin,Bcl-2 and cyclinD1 proteins expression in SKOV3 cells were detected by Western blot.SKOV3 cells were injected into nude mice to establish engrafted ovarian tumor model.The growth of transplanted tumor was measured,and the expression of apoptosis related genes was detected by real-time PCR.Results Compared with control group,Caudatin ould suppress the proliferation of SKOV3 cells and increase the apoptosis in a concentration-dependent way.Caudatin could also reduce the protein expression of DNMT1,Bcl-2 and cyclinD1,but increase Axin.The experiment on tumor growth in the in vivo mice model revealed that Caudatin inhibited the growth of transplanted tumor and the mRNA expression of DNMT1,Bcl-2 and cyclinD1,while increased the Axin mRNA expression level.Conclusion Caudatin can restrain the proliferation and induce apoptosis of ovarian cancer.Its mechanism may be related to regulate DNMT1/β-catenin signaling pathway.
作者
龚建明
林琪
Gong Jianming;Lin Qi(The First Affiliated Hospital of Wenzhou Medical University,Zhejiang 325000,China)
出处
《医学研究杂志》
2021年第10期92-96,共5页
Journal of Medical Research
基金
浙江省温州市公益性科技计划项目(Y20190007)。
